首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠葡萄糖调节蛋白78(GRP78)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GRP78含量。GRP78是重要的内质网分子伴侣。其在细胞应对内质网应激时发挥关键作用，能促进蛋白质正确折叠与组装。异常表达与肿瘤发生、发展及耐药性密切相关。目前研究聚焦其在肿瘤信号通路中的调控机制，有望为肿瘤治疗提供新靶点。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于基础科研中探索GRP78在疾病模型中的动态表达、药物干预效果评估或相关信号通路机制解析，为心血管疾病、癌症生物学等领域的分子机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hspa5 ELISA Kit; Grp78 ELISA Kit; Endoplasmic reticulum chaperone BiP ELISA Kit; EC 3.6.4.10 ELISA Kit; 78 kDa glucose-regulated protein ELISA Kit; GRP-78 ELISA Kit; Binding-immunoglobulin protein ELISA Kit; BiP ELISA Kit; Heat shock protein 70 family protein 5 ELISA Kit; HSP70 family protein 5 ELISA Kit; Heat shock protein family A member 5 ELISA Kit; Immunoglobulin heavy chain-binding protein ELISA Kit
缩写：GRP78
Uniprot No.：P20029
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Tags & Cell Markers
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRP78 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of mouse GRP78 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-98 | ||||
| EDTA plasma (n=4) | 96 | 94-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.8032.9802.8922.739152.1302.2052.1682.0157.51.5471.5111.5291.3763.750.8020.8000.8010.6481.80.5310.5650.5480.3950.90.3710.3850.3780.2250.450.2660.2570.2620.10900.1560.1500.153 | ||||||
货期：3-5 working days
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