首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胃泌素释放肽(GRP)酶联免疫试剂盒，检测血清、血浆、组织匀浆样本中的GRP含量。GRP即胃泌素释放肽，是一种神经递质。它在多种生理过程中发挥作用，还与肿瘤发生发展密切相关。研究机制上，GRP与其受体结合，能激活下游信号通路，促进细胞增殖、抑制凋亡等，因此成为肿瘤等疾病治疗的潜在靶点。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于胃肠功能研究、神经生物学实验及肿瘤相关基础科研领域，满足体外实验中对GRP表达动态的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Grp ELISA Kit; Gastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit
缩写：GRP
Uniprot No.：Q8R1I2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 98 | |
| Range % | 92-102 | ||
| 1:2 | Average % | 98 | |
| Range % | 91-106 | ||
| 1:4 | Average % | 95 | |
| Range % | 89-98 | ||
| 1:8 | Average % | 96 | |
| Range % | 92-104 |
回收率：
| The recovery of mouse GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 89 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 25002.7382.6162.6772.512 12502.1582.0102.0841.919 6251.3901.3681.3791.214 312.50.8660.8860.8760.711 156.250.5600.5420.5510.386 78.1250.3510.3630.3570.192 39.0630.2560.2500.2530.088 00.1620.1670.165 | ||||||
货期：3-5 working days
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