首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人糖皮质激素受体β(GR-β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GR-β含量。GR-β即糖皮质激素受体β，是核受体超家族成员。它能拮抗GR-α转录活性，影响糖皮质激素效应。研究发现其在哮喘等炎症性疾病中异常表达，深入探究其作用机制，可为相关疾病的治疗提供新的靶点与思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于科研领域对GR-β表达水平的精准分析；可为体外研究GR-β在疾病模型中的表达调控、药物干预效果评估以及信号通路机制探索提供可靠工具；实验样本兼容性广泛，支持从基础细胞实验到动物组织研究的多样化需求，操作流程标准化，适用于实验室常规蛋白定量分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
缩写：GR-β
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 88-95 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-96 | ||||||
回收率：
| The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 87 | 83-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 25002.4412.3822.4122.301 12501.6921.7251.7091.598 6251.0211.1031.0620.951 312.50.5630.5390.5510.440 1560.3270.3140.3210.210 780.2450.2580.2520.141 390.1850.1910.1880.077 00.1080.1130.111 | |||||||
货期：3-5 working days
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