首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人谷胱甘肽还原酶（GR）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GSR含量。GSR即谷胱甘肽还原酶，在生物体内负责催化氧化型谷胱甘肽还原为还原型谷胱甘肽，维持细胞内抗氧化防御系统。其研究聚焦于作为药物靶点，针对GSR开发的药物可调节细胞氧化还原状态，用于治疗氧化应激相关疾病如癌症、神经退行性疾病等。试剂盒检测范围为0.625 mU/mL-40 mU/mL，适用于体外研究GR在神经退行性疾病、代谢异常或心血管疾病等病理过程中的表达水平变化，为氧化应激相关分子机制探索提供工具支持。组织匀浆兼容性设计可满足离体组织或动物模型中GR的活性检测需求，为多维度科研实验提供灵活选择。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GLR1 ELISA Kit; GLUR ELISA Kit; Glutathione reductase ELISA Kit; Glutathione reductase mitochondrial ELISA Kit; Glutathione reductase; mitochondrial ELISA Kit; GR ELISA Kit; Gr1 ELISA Kit; GRase ELISA Kit; GRD 1 ELISA Kit; GRD1 ELISA Kit; GSHR_HUMAN ELISA Kit; GSR ELISA Kit; MGC78522 ELISA Kit
缩写：GSR
Uniprot No.：P00390
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 mU/mL-40 mU/mL
灵敏度：0.156 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 96-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human GR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 107 | 101-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected402.8272.7872.8072.648201.8711.8541.8631.704101.1921.1681.1801.02150.6670.6430.6550.4962.50.4660.4550.4610.3021.250.3100.2890.3000.1410.6250.2560.2460.2510.09200.1600.1580.159 | ||||||
货期：3-5 working days
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