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Human granzyme B (GZMB) ELISA Kit

  • 中文名： 人颗粒酶B(GZMB)ELISA Kit
货号: IPD-E08718h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人颗粒酶B(Gzms-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆.样本中的GZMB含量。GZMB是一种重要靶点。它在免疫调节等生理过程中起关键作用，在抗肿瘤免疫里可杀伤肿瘤细胞。研究其机制主要聚焦在它的活性调控、与其他分子相互作用上，清晰其机制有助于研发基于该靶点的免疫治疗药物及方案。试剂盒检测范围为9.38 pg/ml-600 pg/ml.，灵敏度为2.34 pg/ml.。在科研领域，本产品可广泛用于免疫细胞功能研究、肿瘤微环境中细胞毒性效应评估、感染性疾病模型中的免疫应答分析，以及心血管疾病、器官移植排斥反应等相关机制探索，为揭示Gzms-B在病理生理过程中的动态变化提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C11 ELISA Kit; Cathepsin G like 1 ELISA Kit; Cathepsin G-like 1 ELISA Kit; CCPI ELISA Kit; CGL 1 ELISA Kit; CGL1 ELISA Kit; CSP B ELISA Kit; CSPB ELISA Kit; CTLA 1 ELISA Kit; CTLA-1 ELISA Kit; CTLA1 ELISA Kit; CTSGL1 ELISA Kit; Cytotoxic serine protease B ELISA Kit; Cytotoxic T lymphocyte associated serine esterase 1 ELISA Kit; Cytotoxic T lymphocyte proteinase 2 ELISA Kit; Cytotoxic T-lymphocyte proteinase 2 ELISA Kit; Fragmentin 2 ELISA Kit; Fragmentin-2 ELISA Kit; GRAB_HUMAN ELISA Kit; Granzyme 2 ELISA Kit; Granzyme B (granzyme 2; cytotoxic T lymphocyte associated serine esterase 1) ELISA Kit; Granzyme B ELISA Kit; Granzyme-2 ELISA Kit; GranzymeB ELISA Kit; GRB ELISA Kit; Gzmb ELISA Kit; Hlp ELISA Kit; Human lymphocyte protein ELISA Kit; Lymphocyte protease ELISA Kit; Protease; serine; B ELISA Kit; SECT ELISA Kit; T cell serine protease 1 3E ELISA Kit; T cell serine protease 1-3E ELISA Kit; T-cell serine protease 1-3E ELISA Kit
  • 缩写：
    GZMB
  • Uniprot No.：
    P10144
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates.
  • 检测范围：
    9.38 pg/ml-600 pg/ml.
  • 灵敏度：
    2.34 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GZMB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    86-96


    1:2

    Average %

    96


    Range %

    91-102


    1:4

    Average %

    98


    Range %

    94-100


    1:8

    Average %

    93


    Range %

    87-98


  • 回收率：

    The recovery of human GZMB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    90

    86-93


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    600

    3.113

    2.984

    3.048

    2.930


    300

    2.251

    2.207

    2.229

    2.111


    150

    1.612

    1.528

    1.570

    1.452


    75

    1.094

    1.129

    1.111

    0.993


    37.5

    0.655

    0.621

    0.638

    0.519


    18.75

    0.322

    0.343

    0.333

    0.214


    9.38

    0.260

    0.274

    0.267

    0.148


    0

    0.116

    0.121

    0.119




  • 货期：
    3-5 working days

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