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产品描述：人谷胱甘肽硫转移酶pi基因(GSTpi)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、cellsupernates、组织匀浆样本中的GSTP1含量。GSTP1是一种谷胱甘肽 S-转移酶π1。它在细胞解毒、抗氧化应激等过程中发挥重要作用。研究机制上，其活性改变与肿瘤发生、耐药等密切相关，常作为肿瘤诊断标志物及治疗靶点，对其深入研究有助于开发新的抗癌策略。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外研究GSTpi在药物代谢、环境毒理、肿瘤耐药性等领域的表达调控，为科研人员开展氧化应激相关机制探索、化疗药物敏感性评估或新型抗癌药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Deafness ELISA Kit; Deafness X-linked 7 ELISA Kit; DFN7 ELISA Kit; FAEES3 ELISA Kit; Fatty Acid Ethyl Ester Synthase III ELISA Kit; Glutathione S Transferase 3 ELISA Kit; Glutathione S Transferase Pi ELISA Kit; Glutathione S-transferase P ELISA Kit; Glutathione S-transferase pi 1 ELISA Kit; GST class-pi ELISA Kit; GST3 ELISA Kit; GSTP ELISA Kit; Gstp1 ELISA Kit; GSTP1-1 ELISA Kit; GSTP1_HUMAN ELISA Kit; PI ELISA Kit; X linked 7 ELISA Kit
缩写：GSTP1
Uniprot No.：P09211
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GSTpi in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 95-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-95 | |||||
回收率：
| The recovery of human GSTpi spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-95 | ||||
| EDTA plasma (n=4) | 99 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.4742.4582.4662.340501.4791.5881.5341.408250.8150.8800.8480.72212.50.4930.4730.4830.3576.250.3670.3580.3630.2373.120.2630.2530.2580.1321.560.1670.1760.1720.04600.1210.1310.126 | ||||||
货期：3-5 working days
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