首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠颗粒酶B(Gzms-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GZMB含量。GZMB（颗粒酶B）是一种存在于细胞毒性T细胞和NK细胞中的蛋白酶，参与细胞凋亡和免疫反应。其研究机制涉及激活细胞焦亡和裂解细胞膜蛋白，诱导靶细胞死亡，并参与细胞因子处理和细胞外基质蛋白降解。GZMB在肿瘤免疫和炎症反应中发挥重要作用。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域探究免疫细胞活性、细胞毒性反应机制及感染/肿瘤模型中的颗粒酶B动态变化，为免疫学基础研究及药物开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gzmb ELISA kit; Ctla-1 ELISA kit; Ctla1Granzyme B(G,H ELISA kit; EC 3.4.21.79 ELISA kit; CTLA-1 ELISA kit; Cytotoxic cell protease 1 ELISA kit; CCP1 ELISA kit; Fragmentin-2 ELISA kit
缩写：GZMB
Uniprot No.：P04187
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gzms-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 95-106 | ||||||
| 1:2 | Average % | 86 | |||||
| Range % | 82-92 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
回收率：
| The recovery of mouse Gzms-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 96-107 | |||||
| EDTA plasma (n=4) | 93 | 88-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 8002.0762.1432.1101.985 4001.7231.8121.7681.643 2001.2621.2921.2771.152 1000.7830.7960.7900.665 500.4020.4150.4090.284 250.2760.2800.2780.153 12.50.1910.1950.1930.068 00.1240.1260.125 | |||||||
货期：3-5 working days
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