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Pig Hepcidin(HAMP) ELISA kit

  • 中文名: 猪铁调素(HAMP)酶联免疫试剂盒
货号: IPD-EL010124PI
Price: ¥3000
数量:
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产品详情

  • 产品描述:
    猪铁调素(HAMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HAMP含量。试剂盒检测范围为9.38 ng/mL-600 ng/mL,可为猪铁代谢机制研究、营养性缺铁模型构建、疾病状态下铁调素动态变化分析等科研项目提供可靠工具,尤其适用于农业科学、兽医学领域中对铁稳态调控的分子机制探索及生物标志物筛选。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    HAMP ELISA Kit; Hepcidin ELISA Kit
  • 缩写:
    HAMP
  • Uniprot No.:
    Q8MJ80
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    9.38 ng/mL-600 ng/mL
  • 灵敏度:
    2.34 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of pig HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    103


    Range %

    99-109


    1:2

    Average %

    94


    Range %

    89-99


    1:4

    Average %

    98


    Range %

    93-103


    1:8

    Average %

    89


    Range %

    84-95


  • 回收率:

    The recovery of pig HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    91-100


    EDTA plasma (n=4)

    99

    94-104


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    600

    2.751

    2.634

    2.692

    2.543


    300

    2.164

    2.267

    2.215

    2.066


    150

    1.424

    1.368

    1.396

    1.247


    75

    0.859

    0.829

    0.844

    0.695


    37.5

    0.428

    0.421

    0.424

    0.275


    18.75

    0.283

    0.273

    0.278

    0.129


    9.38

    0.201

    0.194

    0.198

    0.049


    0

    0.153

    0.145

    0.149




  • 货期:
    3-5 working days

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