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Human hepcidin(Hepc) ELISA Kit

  • 中文名： 人铁调素(Hepc)ELISA Kit
货号: IPD-E13062h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人铁调素(Hepc)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HAMP含量。HAMP 即肝膜铁转运抑制蛋白，是调节体内铁代谢的关键因子。它能和膜铁转运蛋白结合使其降解，减少铁的吸收和释放。相关研究围绕其在铁稳态维持机制展开，异常表达与多种铁代谢紊乱疾病相关，有望成防治铁代谢疾病的新靶点。适用于体外培养细胞、动物模型组织或体液样本中Hepc表达水平的检测，可满足肝脏生理学、铁代谢调控机制及相关疾病模型的基础研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Hamp ELISA Kit; HEPC ELISA Kit; HEPC_HUMAN ELISA Kit; Hepc20 ELISA Kit; Hepc25 ELISA Kit; HEPCIDIN ELISA Kit; Hepcidin 20 ELISA Kit; Hepcidin 25 ELISA Kit; Hepcidin antimicrobial peptide ELISA Kit; Hepcidin-20 ELISA Kit; Hepcidin25 ELISA Kit; HFE2 ELISA Kit; HFE2B ELISA Kit; LEAP 1 ELISA Kit; LEAP-1 ELISA Kit; LEAP1 ELISA Kit; Liver expressed antimicrobial peptide ELISA Kit; Liver-expressed antimicrobial peptide 1 ELISA Kit; PLTR ELISA Kit; Putative liver tumor regressor ELISA Kit
  • 缩写：
    HAMP
  • Uniprot No.：
    P81172
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    Request Information
  • 灵敏度：
    Request Information
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    0

    0.125

    0.131

    0.128

    0.128


    12.5

    0.287

    0.269

    0.278

    0.278


    37.5

    0.436

    0.428

    0.432

    0.432


    75

    0.697

    0.689

    0.693

    0.693


    150

    1.173

    1.221

    1.197

    1.197


    300

    2.049

    2.053

    2.051

    2.051


  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human hepcidin antibody.

    • Six vials standard (0.5 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated hepcidin antibody (6 ml/bottle) --- Bind to the hepcidin in the samples or standards and react with the substrate to make the solution chromogenic.

    • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.

    • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.

    • One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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