首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬铁调素(HAMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的HAMP含量。HAMP即肝脏抗菌肽，其背景在于它是肝脏产生的一种关键抗菌物质，参与铁代谢和免疫调节。研究机制上，它能结合并降解铁转运蛋白，减少细胞铁外流；还可作为先天免疫效应分子，抵御病原体入侵，在维持机体铁稳态与抗感染中起重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，支持科研人员探究犬类铁代谢相关疾病的分子机制，例如在铁负荷异常模型、营养干预实验或遗传性铁代谢紊乱研究中的应用。实验结果可助力动物生理学、病理学及药理学等领域的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HAMP ELISA Kit; Hepcidin ELISA Kit
缩写：HAMP
Uniprot No.：Q5U9D2
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 96-111 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 90-104 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 90-101 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 82-95 | ||||||
回收率：
| The recovery of dog HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-95 | |||||
| EDTA plasma (n=4) | 107 | 99-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2001.9711.9121.9421.8101001.4821.4771.4801.348500.9760.9880.9820.850250.5460.5560.5510.41912.50.4020.3780.3900.2586.250.2630.2590.2610.1293.120.1780.1720.1750.04300.1310.1330.132 | |||||||
货期：3-5 working days
×
