Mouse Hyaluronic acid,HA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08121m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠透明质酸(HA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HA含量。HA（血凝素）是流感病毒表面的糖蛋白，在病毒感染宿主细胞过程中起关键作用。其可与宿主细胞表面受体结合，介导病毒膜与细胞膜融合。研究HA有助于揭示流感病毒感染机制，为流感疫苗研发、抗病毒药物设计提供重要靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域对HA表达水平的动态监测。在关节炎模型、皮肤再生研究、肿瘤微环境分析及代谢相关疾病机制探索中具有重要研究价值。适用于比较不同病理状态下HA分泌水平差异或评估干预措施对HA代谢的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：HA
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-99
1:200	Average %	93
	Range %	89-101
1:400	Average %	102
	Range %	98-106
1:800	Average %	97
	Range %	92-102

回收率：

The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	93-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected40002.7552.8562.8062.65220002.0272.1282.0781.92410001.4351.4551.4451.2915000.8500.8610.8560.7022500.5260.5150.5210.3671250.3580.3480.3530.19962.50.2670.2610.2640.11000.1540.1530.154