首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人透明质酸(HA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织匀浆样本中的HA含量。HA即血凝素，是流感病毒表面重要糖蛋白。其背景上，它在病毒感染宿主过程中至关重要。研究机制方面，HA可介导病毒与宿主细胞结合及膜融合，促使病毒侵入细胞。对HA研究有助于研发流感疫苗、抗病毒药物等，以应对流感威胁。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研领域可应用于基础医学研究中HA代谢机制探索、药物干预效果评价，或结合动物模型、细胞实验进行跨物种、多组织类型的HA动态监测，为组织再生、炎症调控及疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：HA
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 80-94 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:80 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:160 | Average % | 101 | ||||
| Range % | 97-105 | |||||
回收率：
| The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 93 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.7272.7002.7142.626 52.2312.3452.2882.200 2.51.5581.6551.6071.519 1.250.8310.9010.8660.778 0.6250.4770.4510.4640.376 0.3120.3010.3220.3120.224 0.1560.1890.1740.1820.094 00.0870.0890.088 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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