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产品描述：人透明质酸结合蛋白2(HABP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HABP2含量。HABP2是重要靶点。它是一种丝氨酸蛋白酶，与细胞外基质成分透明质酸结合蛋白相互作用。在纤溶、炎症等生理病理过程起作用。研究多聚焦其参与疾病如癌症、心血管病的机制，有望为开发相关治疗药物提供新方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域对心血管疾病、肿瘤微环境或纤维化疾病等方向的机制研究，可精准分析生物样本中HABP2的浓度变化，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Factor seven activating protease ELISA Kit; Factor seven-activating protease ELISA Kit; Factor VII activating protein ELISA Kit; Factor VII-activating protease ELISA Kit; FSAP ELISA Kit; HABP 2 ELISA Kit; HABP ELISA Kit; Habp2 ELISA Kit; HABP2_HUMAN ELISA Kit; Hepatocyte growth factor activator like protein ELISA Kit; Hepatocyte growth factor activator-like protein ELISA Kit; HGFAL ELISA Kit; Hyaluronan binding protein 2 ELISA Kit; Hyaluronan-binding protein 2 27 kDa light chain alternate form ELISA Kit; Hyaluronic acid binding protein 2 ELISA Kit; PHBP ELISA Kit; Plasma hyaluronan binding protein ELISA Kit; Plasma hyaluronan-binding protein ELISA Kit
缩写：HABP2
Uniprot No.：Q14520
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HABP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 82-96 | |||||
| 1:40 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:160 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of human HABP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 99-105 | ||||
| EDTA plasma (n=4) | 92 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8752.7752.8252.725102.5242.3452.4352.33551.8181.7871.8031.7032.50.9640.9010.9330.8331.250.6020.5860.5940.4940.6250.4000.4250.4130.3130.3120.2050.1950.2000.10000.1010.0980.100 | ||||||
货期：3-5 working days
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