首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：大鼠透明质酸(HA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HA含量。靶点研究是医药创新的关键环节，通过深入疾病分子机制和生物学特性，寻找新的药物靶点，设计精确治疗方案。研究机制包括基因组学、蛋白质组学和生物信息学，以及高通量筛选技术，旨在发现和开发高效、选择性的药物。试剂盒检测范围为4.69 ng/mL-300 ng/mL，适用于探索HA在组织再生、慢性炎症或代谢性疾病中的动态变化，如通过检测肝病模型中的血清HA水平分析纤维化进展，或评估关节炎动物模型中滑膜组织匀浆的HA代谢异常。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：HA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.6562.6782.6672.5051501.8481.8921.8701.708751.2021.2641.2331.07137.50.7630.7780.7710.60918.750.4960.4730.4850.3239.380.3840.3710.3780.2164.690.2750.2980.2870.12500.1610.1630.162 | ||||||
货期：3-5 working days
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