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产品描述：大鼠铁调素(HAMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HAMP含量。HAMP即肝脏抗菌肽，在铁稳态调节和先天免疫中扮演关键角色。研究表明，它通过调控铁转运蛋白功能来调节体内铁代谢。此外，HAMP异常表达与多种疾病密切相关，如铁过载性疾病和贫血等，是相关疾病治疗的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于营养学、铁代谢相关疾病机制研究，如建立铁过载或贫血模型后评估肝源性HAMP的动态变化，或探究炎症、氧化应激等条件下铁调素的调控通路。兼容多种样本类型，支持体内外实验设计本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hamp ELISA Kit; Hepc ELISA Kit; Hepcidin ELISA Kit
缩写：HAMP
Uniprot No.：Q99MH3
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of rat HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2622.2862.2742.12351.6441.7891.7171.5662.51.2161.2561.2361.0851.250.8760.9500.9130.7620.6250.5010.4650.4830.3320.3120.3050.3080.3070.1560.1560.2120.2230.2180.06700.1520.1500.151 | ||||||
货期：3-5 working days
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