首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组氨酰-tRNA合成酶,细胞质(HARS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HARS含量。HARS即组氨酰-tRNA合成酶，是一种参与蛋白质合成的关键酶。研究发现其不仅在蛋白质合成中起作用，还参与了多种疾病相关信号通路的调控，如与神经退行性疾病、肿瘤等发生发展有关，针对它的研究机制或为疾病治疗提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中HARS的蛋白表达分析、病理生理状态下的动态变化监测以及相关分子机制研究，支持生物学、药理学等领域的体外实验需求，为探索HARS在遗传性神经病变、炎症反应或代谢异常等研究场景提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cytoplasmic ELISA Kit; EC 6.1.1.21 ELISA Kit; FLJ20491 ELISA Kit; HARS ELISA Kit; HisRS ELISA Kit; Histidine tRNA ligase, cytoplasmic ELISA Kit; histidine translase ELISA Kit; Histidine tRNA ligase ELISA Kit; Histidine--tRNA ligase ELISA Kit; Histidyl tRNA synthetase ELISA Kit; Histidyl-tRNA synthetase ELISA Kit; HRS ELISA Kit; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds ELISA Kit; SYHC_HUMAN ELISA Kit; USH3B ELISA Kit
缩写：HARS
Uniprot No.：P12081
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human HARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1832.1982.1912.0665001.4251.4381.4321.3072500.8640.8780.8710.7461250.5410.5680.5550.43062.50.3530.3620.3580.23331.250.2770.2680.2730.14815.60.2090.2060.2080.08300.1260.1230.125 | ||||||
货期：3-5 working days
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