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产品描述：人羟基酸氧化酶1(HAO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HAO1含量。HAO1，即羟基酸氧化酶1，是一种FMN依赖的α-羟基酸脱氢酶家族成员，主要在肝脏的过氧化物酶体中氧化甘醇酸生成甘醇酸内酯，同时产生过氧化氢。其表达在氧化应激期间可能下调，以防止过氧化氢在过氧化物酶体中过量生成，可能是一种对氧化损伤的响应机制。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研场景中HAO1的功能研究，如代谢性疾病模型构建、肝肾组织病理分析、药物作用靶点筛选等方向，为探索代谢调控机制及疾病干预策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：(S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
缩写：HAO1
Uniprot No.：Q9UJM8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.69 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 98 | |||||
| Range % | 93-112 | ||||||
| 1:10 | Average % | 107 | |||||
| Range % | 103-109 | ||||||
| 1:20 | Average % | 93 | |||||
| Range % | 90-97 | ||||||
| 1:40 | Average % | 88 | |||||
| Range % | 86-94 | ||||||
回收率：
| The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-106 | |||||
| EDTA plasma (n=4) | 92 | 90-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 12002.1932.3692.2812.097 6001.7001.7101.7051.521 3001.2531.2621.2581.074 1500.8780.8980.8880.704 750.5320.5660.5490.365 37.50.4110.4220.4170.233 18.750.2310.2220.2270.043 00.1770.1900.184 | |||||||
货期：3-5 working days
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