首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人透明质酸合成酶2(HAS2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HAS2含量。HAS2（透明质酸合成酶2）是一种在细胞外基质中合成透明质酸的酶，对维持组织结构和功能至关重要。其作用机制涉及调节细胞增殖、迁移和凋亡，在多种生理和病理过程中发挥重要作用，如骨骼发育、皮肤纤维化、肿瘤生长等。研究HAS2有助于开发针对相关疾病的治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中HA代谢相关机制研究，如关节炎模型中的滑膜组织分析、肿瘤微环境基质重构研究或皮肤创伤修复实验的分子机制探索。为细胞信号转导、组织再生等领域的科研工作提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HAS2; Hyaluronan synthase 2; Hyaluronate synthase 2; Hyaluronic acid synthase 2; HA synthase 2
缩写：HAS2
Uniprot No.：Q92819
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HAS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human HAS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-110 | ||||
| EDTA plasma (n=4) | 87 | 81-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3152.1972.2562.11410001.7961.9011.8491.7075001.3271.4191.3731.2312500.8140.8350.8250.6831250.4810.5030.4920.35062.50.3700.3590.3650.22331.250.2630.2510.2570.11500.1420.1410.142 | ||||||
货期：3-5 working days
×
