关键信息
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基因名
SGK
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简介
SGK protein is a multifunctional serine/threonine protein kinase that complexly controls multiple cellular processes, including ion channel and transporter regulation, enzymatic activity, transcription factors, neuronal excitability, cell growth, proliferation, survival, and migration. and apoptosis. SGK Protein, Human (sf9, His, GST) is the recombinant human-derived SGK protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- 应用
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别名
SGK1; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1
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种属
Human
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表达系统
Baculovirus
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标签
N-8*His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00141
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表达区间
S61-L431
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SGK (serum/glucocorticoid-regulated kinase) is a family of serine/threonine kinases that play a crucial role in various cellular processes, including cell growth, survival, and apoptosis. SGK proteins are closely related to AKT (also known as protein kinase B) and share similar regulatory mechanisms, which are activated by growth factors and hormones. The distinguishing feature of SGK is its response to stress signals such as osmotic shock and glucocorticoids, thus linking it to physiological processes like electrolyte balance and metabolism. Recent research has focused on the role of SGK in various diseases, including cancer, where it has been implicated in promoting tumor cell proliferation, resistance to apoptosis, and enhancement of metastasis. The reconstitution of SGK proteins in laboratory settings has provided valuable insights into its functional dynamics and interactions with other signaling pathways. Understanding the molecular mechanisms underlying SGK activation and its downstream effects has opened up new avenues for therapeutic interventions targeting SGK in conditions such as hypertension, diabetes, and cancer. This ongoing research aims to elucidate the precise roles of SGK in cellular signaling, paving the way for potential drug development and innovative treatment strategies.
