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Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA Kit

  • 中文名: 人肝素结合EGF样生长因子(HBEGF) ELISA kit
货号: IPD-E09716h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人肝素结合EGF样生长因子(HBEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HBEGF含量。HBEGF(肝素结合表皮生长因子样生长因子)是表皮生长因子家族成员,在细胞生长、迁移、分化等多种生理过程中发挥重要作用。其研究机制涉及与特定受体结合激活下游信号通路,参与肿瘤发生发展,在心血管疾病、组织修复等领域也有研究。试剂盒检测范围为62.5 pg/mL-4000 pg/mL,探究HBEGF在炎症反应、肿瘤侵袭性研究或再生医学模型中的动态表达;心血管疾病机制研究、肿瘤微环境分析及干细胞分化调控等基础科研本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA Kit; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA Kit; Diphtheria toxin receptor ELISA Kit; DT R ELISA Kit; DT-R ELISA Kit; DTR ELISA Kit; DTS ELISA Kit; DTSF ELISA Kit; HB-EGF ELISA Kit; HBEGF ELISA Kit; HBEGF_HUMAN ELISA Kit; HEGFL ELISA Kit; Heparin binding EGF like growth factor ELISA Kit; Heparin binding epidermal growth factor ELISA Kit; Heparin binding epidermal growth factor like growth factor ELISA Kit; Heparin-binding EGF-like growth factor ELISA Kit; Proheparin binding EGF like growth factor ELISA Kit

  • 缩写:HBEGF

  • Uniprot No.:Q99075

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:62.5 pg/mL-4000 pg/mL

  • 灵敏度:15.6 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Signal Transduction

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human HB-EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)

    1:1Average %89

    Range %84-93

    1:2Average %86

    Range %82-91

    1:4Average %92

    Range %85-96

    1:8Average %105

    Range %98-109

  • 回收率:

    The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8883-92

    EDTA plasma (n=4)9792-101

















  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected  40002.2142.2672.2412.141  20001.2911.3911.3411.241  10000.6990.7450.7220.622  5000.4180.4120.4150.315  2500.2770.2840.2810.181  1250.1690.1750.1720.072  62.50.1350.1360.1360.036  00.0990.1000.100   







  • 货期:3-5 working days

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