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产品描述:人肝素结合EGF样生长因子(HBEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HBEGF含量。HBEGF(肝素结合表皮生长因子样生长因子)是表皮生长因子家族成员,在细胞生长、迁移、分化等多种生理过程中发挥重要作用。其研究机制涉及与特定受体结合激活下游信号通路,参与肿瘤发生发展,在心血管疾病、组织修复等领域也有研究。试剂盒检测范围为62.5 pg/mL-4000 pg/mL,探究HBEGF在炎症反应、肿瘤侵袭性研究或再生医学模型中的动态表达;心血管疾病机制研究、肿瘤微环境分析及干细胞分化调控等基础科研本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA Kit; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA Kit; Diphtheria toxin receptor ELISA Kit; DT R ELISA Kit; DT-R ELISA Kit; DTR ELISA Kit; DTS ELISA Kit; DTSF ELISA Kit; HB-EGF ELISA Kit; HBEGF ELISA Kit; HBEGF_HUMAN ELISA Kit; HEGFL ELISA Kit; Heparin binding EGF like growth factor ELISA Kit; Heparin binding epidermal growth factor ELISA Kit; Heparin binding epidermal growth factor like growth factor ELISA Kit; Heparin-binding EGF-like growth factor ELISA Kit; Proheparin binding EGF like growth factor ELISA Kit
缩写:HBEGF
Uniprot No.:Q99075
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:62.5 pg/mL-4000 pg/mL
灵敏度:15.6 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HB-EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 89 | |||||
Range % | 84-93 | ||||||
1:2 | Average % | 86 | |||||
Range % | 82-91 | ||||||
1:4 | Average % | 92 | |||||
Range % | 85-96 | ||||||
1:8 | Average % | 105 | |||||
Range % | 98-109 |
回收率:
The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 88 | 83-92 | |||||
EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 40002.2142.2672.2412.141 20001.2911.3911.3411.241 10000.6990.7450.7220.622 5000.4180.4120.4150.315 2500.2770.2840.2810.181 1250.1690.1750.1720.072 62.50.1350.1360.1360.036 00.0990.1000.100 |
货期:3-5 working days
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