Dog Orexin(HCRT) ELISA kit

货号: IPD-EL010230DO

Price： ￥3000

48T 96T

数量：

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产品描述：
犬阿立新(HCRT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HCRT含量。HCRT（食欲素）是一种神经肽，在睡眠-觉醒周期调控等方面发挥重要作用。其背景方面与维持清醒状态相关。研究机制上，它通过与特定受体结合，调节神经元活动，影响多种生理功能，对其研究有助于深入理解睡眠障碍等相关疾病。试剂盒检测范围为1.87 pg/mL-120 pg/mL，适用于神经生物学、动物行为学等领域的科学研究，例如探究犬类睡眠 - 觉醒周期调控机制、代谢疾病模型中的HCRT动态变化，或评估药物干预对HCRT信号通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
HCRT ELISA Kit; OX ELISA Kit; PPOX ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
缩写：
HCRT
Uniprot No.：
Q9GLF6
种属：
Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：
serum, plasma, tissue homogenates
检测范围：
1.87 pg/mL-120 pg/mL
灵敏度：
0.47 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog HCRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	101
	Range %	98-105
1:4	Average %	94
	Range %	89-98
1:8	Average %	103
	Range %	96-107

回收率：

The recovery of dog HCRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-95
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
120	2.176	2.234	2.205	2.101
60	1.659	1.765	1.712	1.608
30	1.193	1.223	1.208	1.104
15	0.793	0.776	0.785	0.681
7.5	0.492	0.483	0.488	0.384
3.75	0.341	0.356	0.349	0.245
1.87	0.242	0.249	0.246	0.142
0	0.103	0.104	0.104

大包装询价