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产品描述：人β氨基己糖苷酶亚基α(HEXA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HEXA含量。HEXA 是一个重要靶点。它相关基因编码的蛋白质对神经系统正常功能维持很关键。研究机制上，聚焦于其在代谢途径中的作用，以及与遗传疾病的关联，旨在通过调控 HEXA 活性，为相关神经系统疾病的治疗提供新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于体外研究HEXA在疾病模型中的表达动态、药物干预对酶活性的影响，以及基因突变与蛋白质功能关联性分析等科研领域，为神经生物学、代谢疾病机制研究提供灵敏可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta hexosaminidase alpha chain precursor ELISA Kit; Beta hexosaminidase subunit alpha ELISA Kit; Beta N acetylhexosaminidase ELISA Kit; Beta N acetylhexosaminidase subunit alpha ELISA Kit; Beta-hexosaminidase A ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexa ELISA Kit; HEXA_HUMAN ELISA Kit; Hexosaminidase A (alpha polypeptide) ELISA Kit; Hexosaminidase A alpha polypeptide ELISA Kit; Hexosaminidase A ELISA Kit; Hexosaminidase subunit A ELISA Kit; MGC99608 ELISA Kit; N acetyl beta glucosaminidase ELISA Kit; N acetyl beta glucosaminidase subunit alpha ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit; TSD ELISA Kit
缩写：HEXA
Uniprot No.：P06865
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HEXA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 94-106 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 87-99 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 85-92 | ||||||
回收率：
| The recovery of human HEXA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 93-102 | |||||
| EDTA plasma (n=4) | 87 | 80-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.2452.1642.2052.053 5001.4071.2951.3511.199 2500.8320.8120.8220.670 1250.4850.4990.4920.340 62.50.3340.3160.3250.173 31.20.2310.2260.2290.077 15.60.1920.1990.1960.044 00.1520.1510.152 | |||||||
货期：3-5 working days
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