首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β氨基己糖苷酶A(β-Hex A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HEXA含量。HEXA是一种溶酶体酶，负责分解GM2神经节苷脂，维持细胞内环境稳定。其突变会导致GM2神经节苷脂在细胞内积累，引发泰萨二氏病等疾病。研究HEXA的机制，旨在开发治疗相关遗传性神经退行性疾病的药物。试剂盒检测范围为3.12 μU/mL-200 μU/mL，适用于研究β-Hex A在代谢性疾病模型小鼠中的表达调控、基因敲除/过表达动物模型的酶活性验证，以及药物干预或病理条件下溶酶体功能的评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
缩写：HEXA
Uniprot No.：P29416
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 μU/mL-200 μU/mL
灵敏度：0.78 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 86-100 | |||||
| 1:80 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-101 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected2002.5422.5172.5302.3861002.0732.1012.0871.943501.4521.4641.4581.314250.9420.9610.9520.80812.50.4160.4020.4090.2656.250.3250.3110.3180.1743.120.2470.2370.2420.09800.1460.1410.144 | ||||||
货期：3-5 working days
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