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产品描述：小鼠肝细胞生长因子激活物(HGFAC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HGFAC含量。HGFAC（肝细胞生长因子激活因子）是一种关键的酶，通过催化肝细胞生长因子（HGF）的激活，将其转化为具有生物活性的异二聚体，参与细胞增殖、迁移和存活等过程。其研究背景涉及细胞信号传导和疾病发生，机制上与HGF的激活和细胞功能密切相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外研究HGFAC在肝脏疾病模型、肿瘤发生机制或组织修复相关实验中的表达动态，为探索HGF/HGFAC信号通路在细胞生物学及分子机制研究提供可靠工具，满足基础科研中对蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hgfac ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
缩写：HGFAC
Uniprot No.：Q9R098
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 98-109 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 93-102 | ||||||
回收率：
| The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 87-98 | |||||
| EDTA plasma (n=4) | 98 | 92-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.5802.4692.5252.35510001.8261.9021.8641.6945001.2031.1831.1931.0232500.6820.6640.6730.5031250.3990.3850.3920.22262.50.2770.2840.2810.11131.250.2030.2090.2060.03600.1690.1710.170 | |||||||
货期：3-5 working days
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