关键信息
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基因名
LOK
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简介
LOK is an important serine/threonine protein kinase that significantly regulates lymphocyte migration by phosphorylating MSN and potentially PLK1. It acts as a negative regulator of MAP3K1/MEKK1. LOK Protein, Human (His) is the recombinant human-derived LOK protein, expressed by E. coli , with N-His labeled tag.
- 应用
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别名
Serine/threonine-protein kinase 10; STK10; LOK
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O94804
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表达区间
R18-E317
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蛋白长度
Partial
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The LOK (Lymphokine-activated killer T-cell-originated protein kinase) is a member of the ste20-like kinase family and plays a crucial role in various cellular processes, including cell proliferation, survival, and apoptosis. Its unique expression pattern in immune cells has attracted significant research attention, particularly in the context of cancer immunotherapy and autoimmune diseases. Studies have shown that LOK is involved in the regulation of T-cell functions, making it a potential target for enhancing immune responses against tumors. Additionally, abnormalities in LOK signaling pathways may contribute to the development of malignancies and autoimmune disorders. The investigation of LOK recombinant proteins can thus provide valuable insights into its functional roles, mechanisms of action, and potential therapeutic applications. By expressing and purifying LOK recombinant proteins, researchers aim to understand its structure-function relationships, identify interaction partners, and elucidate its regulatory pathways. This research not only contributes to our fundamental understanding of immune regulation but also paves the way for novel therapeutic strategies that harness the power of the immune system to fight cancer and other diseases. As the field evolves, the development of specific LOK inhibitors or enhancers could lead to promising advancements in targeted therapies, emphasizing the importance of continued research in this area.
