| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTN4IP1 |
| Uniprot No | Q8WWV3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-396aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVP RGSHMGS TVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNM RSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPG DEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWS AINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDA SELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLK KWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRW AFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGK TVINVV |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RTN4IP1重组蛋白的3篇代表性文献摘要信息：
1. **文献名称**：*RTN4IP1 regulates mitochondrial function and neuronal development through interaction with the reticulon 4 protein*
**作者**：Li X. et al.
**摘要**：本研究通过重组表达纯化RTN4IP1蛋白，发现其与RTN4(又称Nogo-A)直接互作，调控线粒体呼吸链复合物活性，并影响神经元轴突生长。实验证明RTN4IP1重组蛋白的缺失会导致线粒体功能障碍和突触发育异常。
2. **文献名称**：*Structural insights into RTN4IP1-mediated neuroprotection via redox regulation*
**作者**：Wang Y. et al.
**摘要**：文章解析了重组RTN4IP1蛋白的晶体结构，揭示其具有NADPH结合域，可通过调节细胞氧化还原状态保护神经元免受氧化应激损伤。体外实验表明重组蛋白可挽救线粒体膜电位异常。
3. **文献名称**：*Recombinant RTN4IP1 rescues visual impairment in a zebrafish model of optic atrophy*
**作者**：Zhang R. et al.
**摘要**：利用斑马鱼视神经萎缩模型，发现注射重组RTN4IP1蛋白可改善视网膜神经节细胞线粒体运输障碍，并通过激活OPA1蛋白抑制视神经退行性病变，为基因治疗提供潜在策略。
---
**备注**：RTN4IP1相关研究多聚焦于其在线粒体动力学和神经退行性疾病中的作用，重组蛋白研究相对较少。上述文献为假设性示例，实际研究需通过PubMed/Google Scholar以关键词“RTN4IP1 recombinant protein”检索最新成果。
**Background of RTN4IP1 Recombinant Protein**
RTN4IP1 (Reticulon 4 Interacting Protein 1), also known as Neuropathy Target Protein 1 (NTEIP1), is a mitochondrial enzyme encoded by the *RTN4IP1* gene. It interacts with reticulon 4 (RTN4/NOGO-A), a protein involved in neuronal growth inhibition and mitochondrial dynamics. Structurally, RTN4IP1 contains an N-terminal dehydrogenase domain and a C-terminal membrane-binding region, enabling its role in redox reactions and mitochondrial membrane association.
Functionally, RTN4IP1 acts as a NADPH-dependent oxidoreductase, contributing to mitochondrial oxidative phosphorylation (OXPHOS) and energy metabolism. It plays a critical role in neuronal development, synaptic plasticity, and retinal ganglion cell maintenance. Studies highlight its involvement in mitochondrial quality control, including fission-fusion balance and reactive oxygen species (ROS) regulation.
Mutations in *RTN4IP1* are linked to early-onset mitochondrial disorders, particularly Leber’s hereditary optic neuropathy (LHON)-like syndromes and encephalopathies, characterized by vision loss, neurodegeneration, and developmental delays. These pathologies underscore its importance in mitochondrial health and neuronal survival.
Recombinant RTN4IP1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactome, and pathological mechanisms. It serves as a tool for investigating mitochondrial dysfunction in neurodegenerative diseases and screening therapeutic agents targeting OXPHOS deficits. Additionally, recombinant RTN4IP1 aids in structural studies to map functional domains and mutation-induced conformational changes.
Overall, RTN4IP1 recombinant protein is vital for deciphering its role in mitochondrial biology and developing interventions for associated neurological and metabolic disorders.
×
