| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSG1 |
| Uniprot No | Q9BU20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMARPPVPGSVVVPNWHESAEGKEYLAC ILRKNRRRVFGLLERPVLLPPVSIDTASYKIFVSGKSGVGKTALVAKLAG LEVPVVHHETTGIQTTVVFWPAKLQASSRVVMFRFEFWDCGESALKKFDH MLLACMENTDAFLFLFSFTDRASFEDLPGQLARIAGEAPGVVRMVIGSKF DQYMHTDVPERDLTAFRQAWELPLLRVKSVPGRRLADGRTLDGRAGLADV AHILNGLAEQLWHQDQVAAGLLPNPPESAPE |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RSG1重组蛋白的3篇示例参考文献(内容为虚构，仅供格式参考)：
1. **文献名称**：*Recombinant RSG1 Protein Expression and Its Role in Plant Stress Response*
**作者**：Chen L, et al.
**摘要**：研究利用大肠杆菌系统表达RSG1重组蛋白，证实其在拟南芥中通过调控赤霉素信号通路增强植物对盐胁迫的抗性。
2. **文献名称**：*Structural Characterization of RSG1 Recombinant Protein in GTPase Activation*
**作者**：Yamamoto K, et al.
**摘要**：通过X射线晶体学解析RSG1重组蛋白的三维结构，揭示其通过GTPase结构域激活下游信号分子，参与细胞周期调控的分子机制。
3. **文献名称**：*RSG1 Recombinant Protein Inhibits Tumor Metastasis via Modulating Rho Signaling*
**作者**：Wang H, et al.
**摘要**：体外实验表明，纯化的RSG1重组蛋白可抑制癌细胞迁移，机制涉及与Rho蛋白相互作用并阻断其信号传导通路，为癌症治疗提供潜在靶点。
注：以上文献为示例，实际引用请以真实出版物为准。
RSG1 (Responsive to Stimulation by Genistein 1) is a protein encoded by the *RSG1* gene, initially identified for its role in cellular responses to genistein, a plant-derived isoflavone with estrogen-like activity. Belonging to the Regulator of G-protein Signaling (RGS) family, RSG1 is implicated in modulating G-protein-coupled receptor (GPCR) signaling pathways, which regulate diverse physiological processes, including cell proliferation, differentiation, and apoptosis. Unlike canonical RGS proteins that primarily act as GTPase-activating proteins (GAPs) to terminate Gα subunit activity, RSG1 lacks the conserved RGS domain, suggesting a distinct regulatory mechanism. Its structure includes a conserved N-terminal domain and a C-terminal region with potential phosphorylation sites, hinting at post-translational modifications influencing its function.
Recombinant RSG1 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active forms for research. Studies highlight its involvement in cancer progression, as dysregulated RSG1 expression correlates with tumorigenesis in certain cancers, possibly through interactions with signaling pathways like MAPK/ERK or Wnt/β-catenin. Additionally, RSG1 may influence immune responses and neuronal development, though mechanistic insights remain limited. Its responsiveness to genistein links it to phytoestrogen-mediated effects, relevant in hormone-related diseases and therapeutics. Recombinant RSG1 enables exploration of its molecular interactions, structural features, and therapeutic potential, though further research is needed to fully elucidate its physiological and pathological roles. This protein represents a promising target for understanding GPCR-independent RGS functions and developing targeted therapies.
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