| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS7 |
| Uniprot No | P62081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMFSSSAKIVKPNGEKPDEFESGISQA LLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQ VRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVH DAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGV YKKLTGKDVNFEFPEFQL |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS7重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**：*Expression and Purification of Recombinant Ribosomal Protein S7 for Structural Studies*
**作者**：Smith J et al.
**摘要**：该研究利用大肠杆菌表达系统成功表达并纯化了重组RPS7蛋白，旨在通过X射线晶体学解析其三维结构，为核糖体小亚基的组装机制提供结构基础。
2. **文献名称**：*Functional Analysis of Recombinant RPS7 in Ribosome Assembly*
**作者**：Lee H et al.
**摘要**：通过体外重组实验，证明重组RPS7在核糖体小亚基组装中起关键作用，特别是对18S rRNA的正确折叠和稳定化具有不可或缺的功能。
3. **文献名称**：*Recombinant RPS7 as a Potential Therapeutic Target in Diamond-Blackfan Anemia*
**作者**：Garcia M et al.
**摘要**：该研究利用重组RPS7蛋白进行功能互补实验，揭示了其在Diamond-Blackfan贫血症(与RPS7基因突变相关)中的潜在治疗价值，为靶向治疗提供了实验依据。
(注：以上文献信息为示例，实际引用时请核实具体文献内容。)
**Background of RPS7 Recombinant Protein**
Ribosomal protein S7 (RPS7) is a critical component of the 40S ribosomal subunit, playing a dual role in ribosome biogenesis and extraribosomal functions. As part of the small ribosomal subunit, RPS7 contributes to the assembly and stability of the ribosome, ensuring accurate translation of mRNA into proteins. Beyond its canonical role in protein synthesis, RPS7 is implicated in diverse cellular processes, including DNA repair, apoptosis, and cell cycle regulation. Studies have highlighted its interaction with tumor suppressors (e.g., p53) and oncogenic signaling pathways, linking dysregulated RPS7 expression to cancers and developmental disorders.
Recombinant RPS7 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, to produce high-purity, functional protein for research. The recombinant form retains structural and functional integrity, enabling mechanistic studies on ribosome assembly, translational fidelity, and disease-related pathways. Its applications span structural biology (e.g., cryo-EM studies of ribosome architecture), drug discovery (screening for anticancer agents targeting RPS7-interacting networks), and diagnostics (biomarker development for RPS7-associated pathologies).
Recent advances in recombinant technology have also facilitated the exploration of RPS7's non-ribosomal roles, such as its involvement in viral infection responses and stress signaling. These insights underscore RPS7 as a multifunctional protein with therapeutic and diagnostic potential, driving ongoing research into its molecular interactions and disease relevance.
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