| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS24 |
| Uniprot No | P62847-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMNDTVTI RTRKFMTNRL LQRKQMVIDV LHPGKATVPK TEIREKLAKM YKTTPDVIFV FGFRTHFGGG KTTGFGMIYD SLDYAKKNEP KHRLARHGLY EKKKTSRKQR KERKNRMKKV RGTAKANVGA GKK |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS24重组蛋白的虚构参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**: *Functional characterization of recombinant human RPS24 in ribosome biogenesis*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了重组人源RPS24蛋白，发现其在体外核糖体亚基组装中起关键作用。实验表明RPS24缺失会导致核糖体RNA加工异常，提示其与先天性骨髓衰竭综合征的潜在关联。
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2. **文献名称**: *RPS24 mutations and recombinant protein rescue in Diamond-Blackfan anemia models*
**作者**: Chen L, et al.
**摘要**: 作者利用昆虫细胞表达系统制备了重组RPS24蛋白，并在斑马鱼疾病模型中验证其功能。结果显示，外源性RPS24可部分恢复突变体造血干细胞分化缺陷，为基因治疗提供实验依据。
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3. **文献名称**: *Structural analysis of recombinant RPS24 and its interaction with 18S rRNA*
**作者**: Wang X, et al.
**摘要**: 通过X射线晶体学解析了重组RPS24蛋白的三维结构，揭示了其与18S rRNA特异性结合的分子机制。研究为靶向核糖体蛋白的药物设计提供了结构基础。
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4. **文献名称**: *High-yield production of RPS24 in mammalian expression systems for functional studies*
**作者**: Gupta R, et al.
**摘要**: 优化了哺乳动物细胞中重组RPS24的高效表达方案，并证明其与天然蛋白具有相同的翻译活性。该方法为研究RPS24在癌症中的异常调控机制提供了可靠工具。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
RPS24 (Ribosomal Protein S24) is a component of the 40S ribosomal subunit, playing a critical role in ribosome assembly and protein synthesis. As part of the eukaryotic translation machinery, it contributes to the structural stability of the ribosome and ensures accurate decoding of mRNA. Dysregulation or mutations in RPS24 have been linked to genetic disorders such as Diamond-Blackfan anemia (DBA), a rare bone marrow failure syndrome characterized by defective erythropoiesis. Studies suggest that RPS24 haploinsufficiency disrupts ribosomal biogenesis, leading to cellular stress and apoptosis in hematopoietic progenitor cells.
Recombinant RPS24 protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cell cultures) to produce purified, biologically active forms for research. This protein typically retains post-translational modifications when expressed in eukaryotic systems, enhancing its functional relevance. Researchers utilize recombinant RPS24 to investigate ribosome assembly dynamics, translational fidelity, and disease mechanisms in DBA. It also serves as a tool for screening therapeutic agents aimed at modulating ribosomal function or alleviating ribosomalopathies. Structural studies of recombinant RPS24. often combined with cryo-EM or X-ray crystallography, provide insights into its interactions within the ribosome and potential mutation-induced conformational changes. Additionally, it aids in studying cross-species conservation of ribosomal proteins, highlighting evolutionary adaptations in protein synthesis pathways. The development of recombinant RPS24 has thus advanced both basic and translational research in hematopoiesis, cancer biology, and rare genetic disorders.
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