| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS18 |
| Uniprot No | P62269 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSLVIPE KFQHILRVLN TNIDGRRKIA FAITAIKGVG RRYAHVVLRK ADIDLTKRAG ELTEDEVERV ITIMQNPRQY KIPDWFLNRQ KDVKDGKYSQ VLANGLDNKL REDLERLKKI RAHRGLRHFW GLRVRGQHTK TTGRRGRTVG VSKKK |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS18重组蛋白的3篇代表性文献示例(注：以下内容为模拟生成，非真实文献，仅供格式参考)：
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1. **文献名称**：*Structural Characterization of Recombinant Human RPS18 and Its Role in Ribosome Assembly*
**作者**：Smith A, et al.
**摘要**：该研究通过大肠杆菌表达系统成功纯化了重组人源RPS18蛋白，并利用X射线晶体学解析了其三维结构。研究发现RPS18在核糖体小亚基组装中起关键作用，其C端结构域与18S rRNA的特定区域相互作用，为理解核糖体生物合成机制提供了结构基础。
2. **文献名称**：*RPS18 Overexpression Promotes Tumor Progression via mTOR Signaling in Colorectal Cancer*
**作者**：Zhang Y, et al.
**摘要**：通过重组RPS18蛋白的功能实验，作者发现其在结直肠癌细胞中异常高表达可激活mTOR通路，促进细胞增殖和迁移。研究揭示了RPS18作为潜在癌症治疗靶点的可能性。
3. **文献名称**：*Recombinant RPS18 as a Novel Biomarker for Diamond-Blackfan Anemia Diagnosis*
**作者**：Lee H, et al.
**摘要**：该研究开发了重组RPS18蛋白的ELISA检测方法，发现其在Diamond-Blackfan贫血(DBA)患者中表达水平显著降低。实验表明，RPS18功能缺失导致红细胞生成障碍，为DBA的分子诊断提供了新策略。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science检索关键词“RPS18 recombinant protein”“ribosomal protein S18”等获取真实文献。如需具体文献，可提供更详细的研究方向(如结构、疾病、技术方法等)。
**Background of RPS18 Recombinant Protein**
RPS18 (Ribosomal Protein S18) is a key component of the 40S ribosomal subunit, essential for mRNA translation in eukaryotic cells. As part of the ribosome, it plays a structural role in maintaining the subunit's integrity and facilitates the decoding of genetic information during protein synthesis. Beyond its canonical function, RPS18 has been implicated in extraribosomal roles, including cell cycle regulation, apoptosis, and interactions with viral or oncogenic proteins, suggesting its involvement in disease pathways such as cancer.
The recombinant RPS18 protein is produced using biotechnological methods, often via expression in bacterial (e.g., *E. coli*) or eukaryotic systems, followed by purification techniques like affinity chromatography. This engineered protein retains the functional domains of native RPS18. enabling studies on ribosome assembly, translational fidelity, and protein-RNA interactions. Its high purity and activity make it valuable for *in vitro* assays, structural analyses (e.g., X-ray crystallography), and screening for therapeutic agents targeting translation-related disorders.
Mutations or dysregulation of RPS18 are linked to human diseases, including Diamond-Blackfan anemia (DBA), a rare bone marrow failure syndrome, highlighting its role in hematopoiesis. Research on recombinant RPS18 also aids in understanding viral mechanisms, as some pathogens exploit ribosomal proteins for replication. Overall, RPS18 recombinant protein serves as a critical tool for dissecting ribosome biology and developing treatments for ribosomalopathies or cancers associated with translation defects.
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