| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS10 |
| Uniprot No | P46783 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMLMPKKNRIAIYELLFKEGVMVAKKDV HMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQ YLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTY RRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS10重组蛋白的3篇参考文献，按研究内容分类简要概括：
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1. **文献名称**：*Recombinant expression and functional characterization of human ribosomal protein S10 in Escherichia coli*
**作者**：Zhang L. et al.
**摘要**：该研究通过构建pET载体在大肠杆菌中高效表达人源RPS10重组蛋白，优化了纯化条件并验证其与核糖体RNA的结合活性，为后续功能研究提供基础工具。
2. **文献名称**：*Structural analysis of RPS10 in ribosome biogenesis using recombinant protein crosslinking*
**作者**：Kim S., Park J.H.
**摘要**：利用重组RPS10蛋白进行交联实验和质谱分析，揭示了其在核糖体组装过程中与18S rRNA及相邻蛋白的相互作用位点，阐明其结构功能关系。
3. **文献名称**：*RPS10 overexpression promotes tumor cell proliferation via mTOR signaling: Evidence from recombinant protein-based assays*
**作者**：Chen X. et al.
**摘要**：通过体外表达RPS10重组蛋白，发现其过表达激活mTOR通路并加速肿瘤细胞周期进程，提示其在癌症中的潜在治疗靶点价值。
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**说明**：以上文献为示例，实际文献需通过PubMed或Web of Science检索关键词“RPS10 recombinant protein”或结合具体研究领域筛选。建议补充实验用途(如结构、疾病机制等)以缩小检索范围。
The ribosomal protein S10 (RPS10) is a critical component of the 40S ribosomal subunit, playing a central role in ribosome biogenesis and protein synthesis. As part of the small ribosomal subunit, RPS10 contributes to rRNA processing, ribosome assembly, and the stabilization of ribosomal structure. Beyond its canonical role in translation, RPS10 has been implicated in extra-ribosomal functions, including cell cycle regulation, apoptosis, and interactions with viral or cellular RNAs. Dysregulation of RPS10 expression has been associated with various cancers, neurodegenerative disorders, and developmental defects, highlighting its broader biological significance.
Recombinant RPS10 protein is engineered using genetic cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The protein is often purified via affinity tags (e.g., His-tag) followed by chromatography, yielding high-purity products for functional studies. Recombinant RPS10 serves as a vital tool for investigating ribosome assembly mechanisms, studying its interactions with RNA or other ribosomal proteins, and elucidating its non-canonical roles in disease pathways. Researchers utilize it in structural analyses (e.g., X-ray crystallography, cryo-EM), in vitro binding assays, and cellular studies to explore its impact on translation fidelity or stress responses. Additionally, it supports therapeutic research, such as screening for inhibitors targeting ribosome dysfunction in cancer or validating RPS10 as a diagnostic biomarker. The development of recombinant RPS10 has significantly advanced our understanding of ribosome biology and its intersection with human diseases.
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