| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPN2 |
| Uniprot No | P04844 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-540aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAA QASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIV EEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPV VVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIAN TVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVA EPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPN2重组蛋白的3篇虚构参考文献示例(仅供格式参考)：
1. **文献名称**：*RPN2 regulates N-linked glycosylation and cancer cell viability through OST complex assembly*
**作者**：Tanaka, K. et al.
**摘要**：本研究利用重组RPN2蛋白解析其在寡糖转移酶(OST)复合体组装中的作用，发现RPN2缺失会破坏复合体稳定性，导致蛋白质N-糖基化缺陷，进而诱导内质网应激并抑制癌细胞增殖。
2. **文献名称**：*Recombinant RPN2 binds to ER chaperones and modulates substrate recognition in glycosylation*
**作者**：Chen, L. & Wang, H.
**摘要**：通过体外重组RPN2蛋白实验，证明其与内质网伴侣蛋白BiP/GRP78相互作用，调控底物蛋白的识别与糖基化效率，为靶向糖基化通路的癌症治疗提供新思路。
3. **文献名称**：*RPN2-mediated glycosylation promotes chemoresistance in colorectal cancer via stabilizing ABC transporters*
**作者**：Gupta, S. et al.
**摘要**：研究发现，过表达重组RPN2蛋白可增强结直肠癌细胞中ABC转运蛋白的糖基化修饰，提高其膜稳定性，导致化疗药物外排增加和耐药性;敲低RPN2则逆转此效应。
(注：以上文献为模拟示例，实际文献需通过学术数据库检索确认。)
**Background of RPN2 Recombinant Protein**
RPN2 (Ribophorin II) is a key component of the oligosaccharyltransferase (OST) complex, a multisubunit enzyme machinery located in the endoplasmic reticulum (ER). It plays a critical role in N-glycosylation, a post-translational modification essential for protein folding, quality control, and cellular trafficking. As part of the OST complex, RPN2 facilitates the transfer of preassembled oligosaccharides from lipid-linked precursors to asparagine residues on nascent polypeptides, a process vital for the structural and functional integrity of secreted and membrane-bound proteins.
The recombinant RPN2 protein is engineered through heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling large-scale production for research and therapeutic applications. Its recombinant form retains functional domains, including the transmembrane region and luminal domain, critical for interacting with other OST subunits (e.g., ribophorin I, STT3) and substrate recognition.
Studies on RPN2 have highlighted its involvement in diseases, particularly cancer and neurodegenerative disorders. Dysregulation of N-glycosylation due to RPN2 abnormalities is linked to tumor progression, drug resistance, and impaired neuronal protein homeostasis. Recombinant RPN2 serves as a tool to dissect glycosylation mechanisms, screen glycosylation-modulating drugs, and develop biologics targeting glycosylation-related pathologies. Additionally, it aids in structural studies to map OST complex assembly and catalytic sites.
Overall, RPN2 recombinant protein bridges fundamental glycosylation research with translational applications, offering insights into cellular processes and therapeutic strategies for glycosylation-associated diseases.
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