纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | RPL8 |
Uniprot No | P62917 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-257aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN |
预测分子量 | 30 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL8重组蛋白的假设性参考文献示例(仅供格式和内容参考,实际文献需通过学术数据库查询):
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1. **标题**: *Structural characterization of recombinant human RPL8 and its role in ribosome biogenesis*
**作者**: Smith, J., et al.
**摘要**: 本研究通过大肠杆菌表达系统纯化了重组人源RPL8蛋白,利用X射线晶体学解析其三维结构,揭示了RPL8在核糖体大亚基组装中的关键作用,并发现其与28S rRNA的特定结构域相互作用。
2. **标题**: *Optimization of recombinant RPL8 production in yeast for functional studies*
**作者**: Lee, H., et al.
**摘要**: 通过酵母表达系统优化重组RPL8的产量和可溶性,验证其在体外蛋白质合成中的功能,并证明其缺失会导致核糖体翻译效率显著降低。
3. **标题**: *RPL8 interacts with hepatitis C virus RNA to facilitate viral replication*
**作者**: Zhang, Y., et al.
**摘要**: 利用重组RPL8蛋白进行RNA结合实验,发现其直接结合丙型肝炎病毒(HCV)的5'非编码区,调控病毒RNA的稳定性,为抗病毒靶点研究提供依据。
4. **标题**: *Recombinant RPL8 as a potential biomarker in colorectal cancer*
**作者**: Wang, L., et al.
**摘要**: 通过重组RPL8蛋白制备抗体,分析结直肠癌患者组织中RPL8的异常高表达,证实其通过激活mTOR信号通路促进肿瘤细胞增殖。
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**注意**:以上文献为示例,实际研究中请通过PubMed、Web of Science等平台检索真实发表的论文。如需具体文献支持,建议结合关键词“RPL8 recombinant protein”“ribosomal protein L8”等进行精准查询。
**Background of Recombinant RPL8 Protein**
RPL8 (Ribosomal Protein L8) is a component of the 60S ribosomal subunit, playing a critical role in protein synthesis by facilitating ribosome assembly and mRNA translation. As part of the conserved ribosomal protein family, RPL8 is encoded by the *RPL8* gene in humans and shares structural homology across eukaryotes. Beyond its canonical ribosomal function, RPL8 has been implicated in extraribosomal roles, including interactions with viral RNAs, modulation of cellular stress responses, and regulation of gene expression through binding to specific mRNAs or non-coding RNAs.
Recombinant RPL8 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells) to enable functional and structural studies. Its production typically involves cloning the *RPL8* gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). The recombinant protein retains the ability to integrate into ribosomal subunits and participate in translation, making it valuable for studying ribosome biogenesis, translation fidelity, and interactions with cellular or viral components.
Research highlights RPL8's potential roles in disease. For example, dysregulation of RPL8 expression has been linked to cancers, where it may influence tumor progression by modulating oncogenic pathways or ribosome-mediated stress adaptation. Additionally, RPL8 interacts with viruses such as hepatitis C, suggesting a role in viral replication.
Despite advances, challenges remain in understanding its extraribosomal functions and post-translational modifications. Recombinant RPL8 serves as a key tool for dissecting these mechanisms, offering insights into ribosome biology and therapeutic targeting in diseases linked to translational dysregulation.
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