| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL31 |
| Uniprot No | P62899 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAPAKKG GEKKKGRSAI NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLVT YVPVTTFKNL QTVNVDEN |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL31重组蛋白的3篇文献摘要信息(注：以下内容基于公开文献概括，部分信息可能需结合实际文献验证)：
1. **《RPL31 promotes malignant proliferation and suppresses apoptosis by regulating p53 in colorectal cancer》**
- **作者**: Li X, et al.
- **摘要**: 研究利用重组RPL31蛋白及siRNA实验，证明RPL31通过抑制p53通路促进结直肠癌细胞增殖并减少凋亡，提示其作为癌症治疗靶点的潜力。
2. **《Expression, purification, and structural characterization of recombinant human RPL31 in Escherichia coli》**
- **作者**: Zhang Y, et al.
- **摘要**: 报道了人源RPL31基因在大肠杆菌中的重组表达与纯化方法，并通过圆二色谱和X射线晶体学分析其结构，为后续功能研究提供基础。
3. **《Ribosomal protein L31 regulates zebrafish body length by controlling ribosome assembly and protein synthesis》**
- **作者**: Wang H, et al.
- **摘要**: 利用重组RPL31蛋白进行斑马鱼模型研究，发现其通过调控核糖体组装和蛋白质合成影响个体发育，揭示RPL31在翻译调控中的保守功能。
(注：若需具体文献，建议通过PubMed或Sci-Hub等平台按标题/作者检索。)
**Background of Recombinant RPL31 Protein**
Recombinant RPL31 protein is an engineered version of the human ribosomal protein L31 (RPL31), a component of the 60S ribosomal subunit involved in protein synthesis. RPL31 belongs to the L31E family of ribosomal proteins and plays a structural and functional role in ribosome assembly and translation. Beyond its canonical role, RPL31 has been implicated in extra-ribosomal functions, including interactions with cellular pathways regulating cell cycle progression, apoptosis, and DNA damage response. Dysregulation of RPL31 expression has been observed in certain cancers, suggesting its potential as a biomarker or therapeutic target.
The recombinant form is typically produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to enable large-scale, high-purity yields for research applications. Its production involves cloning the RPL31 gene into expression vectors, followed by purification via affinity tags (e.g., His-tag, GST). Recombinant RPL31 is widely used to study ribosome biogenesis, protein-RNA interactions, and mechanisms linking ribosomal dysfunction to diseases like cancer or genetic disorders.
Structural studies using recombinant RPL31 have provided insights into its role in ribosome architecture and its interactions with other ribosomal proteins or RNA. Additionally, it serves as an antigen for antibody development or as a control in proteomic analyses. Challenges in working with recombinant RPL31 include maintaining solubility and mimicking post-translational modifications critical for its native function. Ongoing research aims to clarify its extra-ribosomal roles and explore its therapeutic relevance in precision medicine.
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