| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL18A |
| Uniprot No | Q02543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-176aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGS MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSGT HNMYREYRDL TTAGAVTQCY RDMGARHRAR AHSIQIMKVE EIAASKCRRP AVKQFHDSKI KFPLPHRVLR RQHKPRFTTK RPNTFF |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL18A重组蛋白的3篇参考文献示例(内容为模拟虚构，供参考)：
1. **"Recombinant RPL18A promotes hepatocellular carcinoma progression via mTOR signaling activation"**
- 作者：Zhang Y, et al.
- 摘要：研究通过重组RPL18A蛋白体外实验，发现其与mTOR通路蛋白相互作用，促进肝癌细胞增殖和迁移，提示RPL18A可能作为癌症治疗的潜在靶点。
2. **"Expression and structural characterization of human RPL18A in a prokaryotic system"**
- 作者：Li H, Wang X.
- 摘要：利用大肠杆菌系统成功表达并纯化重组RPL18A蛋白，通过X射线晶体学解析其三维结构，为研究核糖体组装机制提供了分子基础。
3. **"RPL18A recombinant protein interacts with viral RNA to facilitate enterovirus replication"**
- 作者：Chen L, et al.
- 摘要：发现重组RPL18A蛋白可与肠道病毒RNA结合，增强病毒复制效率，揭示了核糖体蛋白在病毒感染中的非经典功能。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词**"RPL18A recombinant protein"**或**"RPL18A expression and function"**获取最新研究。
**Background of RPL18A Recombinant Protein**
RPL18A (Ribosomal Protein L18a) is a key component of the 60S ribosomal subunit, playing a critical role in protein biosynthesis by facilitating the assembly and stability of ribosomal structures. Encoded by the *RPL18A* gene, this protein is evolutionarily conserved across eukaryotes, underscoring its essential function in cellular viability. As part of the ribosomal machinery, RPL18A contributes to mRNA decoding, peptidyl transfer, and ribosome biogenesis, interacting with rRNA and other ribosomal proteins to maintain translational fidelity.
The recombinant RPL18A protein is engineered using biotechnological methods, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production involves cloning the *RPL18A* gene into expression vectors, followed by purification via affinity chromatography (e.g., His-tag systems). Recombinant RPL18A retains functional properties, enabling its use in structural studies (e.g., cryo-EM or X-ray crystallography) to elucidate ribosome architecture.
Beyond basic research, RPL18A has biomedical relevance. Dysregulation of ribosomal proteins, including RPL18A, is linked to cancers, neurodegenerative disorders, and ribosomopathies. Overexpression of RPL18A has been observed in certain tumors, suggesting potential roles in oncogenesis or as a therapeutic target. Recombinant RPL18A also aids in studying ribosome-associated diseases, antiviral mechanisms (e.g., interactions with viral RNA), and drug screening for compounds targeting ribosomal function.
In summary, recombinant RPL18A serves as a vital tool for dissecting ribosomal biology and disease mechanisms, bridging molecular insights with translational applications.
×
