| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPIA |
| Uniprot No | P49247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQRPGPFSTLYGRVLAPLPGRAGGAASGGG GNSWDLPGSHVRLPGRAQSGTRGGAGNTSTSCGDSNSICPAPSTMSKAEE AKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIP TSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLT QEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVS QKFGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPG VVDTGLFINMAERVYFGMQDGSVNMREKPFC |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPIA(核糖-5-磷酸异构酶A)重组蛋白研究的3篇示例文献摘要，供参考(注：文献为虚构示例，实际引用请查询数据库)：
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1. **文献名称**：*Heterologous Expression and Characterization of Recombinant Ribose-5-Phosphate Isomerase A (RPIA) from Escherichia coli*
**作者**：Chen et al.
**摘要**：本研究通过在大肠杆菌中克隆并表达RPIA重组蛋白，优化了表达条件(如诱导温度、IPTG浓度)，并采用亲和层析技术纯化获得高纯度蛋白。酶活实验表明重组RPIA具有催化核糖-5-磷酸与核酮糖-5-磷酸异构化的活性，为后续酶学机制研究奠定基础。
2. **文献名称**：*Structural Insights into RPIA Function in the Pentose Phosphate Pathway*
**作者**：Kimura et al.
**摘要**：通过X射线晶体学解析了人源RPIA重组蛋白的三维结构(分辨率2.1Å)，揭示了其活性位点关键氨基酸残基(如Arg86、His110)在底物结合中的作用，并基于突变体实验提出其催化机制可能与质子转移相关。
3. **文献名称**：*Role of RPIA in Cancer Cell Metabolism: A Study Using Recombinant Protein Overexpression*
**作者**：Gupta et al.
**摘要**：在肝癌细胞中过表达重组RPIA蛋白，发现其通过增强磷酸戊糖途径活性显著提高NADPH水平，促进肿瘤细胞增殖和抗氧化应激能力，提示RPIA可能成为癌症治疗的潜在靶点。
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**建议**：实际文献可通过PubMed、Web of Science等平台，以关键词“RPIA recombinant protein”“Ribose-5-phosphate isomerase expression”或结合研究领域(如“cancer”“structural analysis”)检索获取。
**Background of RPIA Recombinant Protein**
Ribose-5-phosphate isomerase A (RPIA) is a key enzyme in the pentose phosphate pathway (PPP), a metabolic pathway critical for nucleotide synthesis, redox balance, and carbohydrate metabolism. RPIA catalyzes the interconversion of ribulose-5-phosphate (Ru5P) and ribose-5-phosphate (R5P), a reversible step that bridges glycolysis with nucleotide biosynthesis and NADPH production. Dysregulation of RPIA has been implicated in metabolic disorders, cancer progression, and neurodegenerative diseases, highlighting its biological and clinical significance.
Recombinant RPIA protein refers to the enzymatically active form of RPIA produced through genetic engineering techniques. By inserting the *RPIA* gene into expression vectors (e.g., bacterial, yeast, or mammalian systems), researchers can produce large quantities of purified RPIA protein for functional and structural studies. This recombinant approach ensures high purity, consistency, and scalability, addressing challenges associated with isolating the protein from native tissues.
RPIA recombinant protein has become a vital tool for investigating PPP dynamics, particularly in contexts like cancer metabolism. Cancer cells often upregulate PPP enzymes, including RPIA, to meet their heightened demand for nucleotides and antioxidants. Studies using recombinant RPIA have revealed its role in sustaining tumor proliferation and chemoresistance, making it a potential therapeutic target. Additionally, mutations in *RPIA* are linked to rare metabolic disorders, such as ribose-5-phosphate isomerase deficiency, which causes developmental delays and neurological impairments. Recombinant RPIA aids in characterizing these mutations and screening corrective compounds.
In drug discovery, recombinant RPIA enables high-throughput assays to identify inhibitors or activators that modulate PPP flux. Structural analyses using crystallography or cryo-EM, facilitated by recombinant protein, provide insights into its catalytic mechanisms and allosteric regulation. Beyond research, recombinant RPIA holds promise for enzyme replacement therapies in genetic disorders, though this application remains exploratory.
Overall, RPIA recombinant protein serves as a cornerstone for advancing our understanding of metabolic regulation and its translational implications in health and disease.
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