| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROBLD3 |
| Uniprot No | Q9Y2Q5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMLRPKALTQVLSQANTGGVQSTLLLN NEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDC MEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQVAAS |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的关于“ROBLD3重组蛋白”的参考文献示例(实际文献可能需要进一步验证或调整关键词)：
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1. **文献名称**: "Cloning and Functional Characterization of Recombinant ROBLD3 Protein in E. coli"
**作者**: Zhang, Y. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化ROBLD3重组蛋白，通过圆二色谱分析证实其正确折叠。实验表明，重组ROBLD3能特异性结合细胞表面受体，可能在神经突触形成中发挥作用。
2. **文献名称**: "ROBLD3 Recombinant Protein Inhibits Tumor Cell Migration via Slit-Robo Signaling"
**作者**: Li, H., Wang, X.
**摘要**: 利用哺乳动物表达系统制备ROBLD3重组蛋白，发现其通过Slit-Robo信号通路抑制乳腺癌细胞的迁移，提示其作为潜在抗癌靶点的价值。
3. **文献名称**: "Structural Insights into ROBLD3-Ligand Interactions by Cryo-EM"
**作者**: Smith, J. et al.
**摘要**: 通过冷冻电镜解析ROBLD3重组蛋白与配体复合物的三维结构，揭示了其结合域的关键氨基酸残基，为设计小分子抑制剂提供结构基础。
4. **文献名称**: "Optimization of ROBLD3 Recombinant Protein Purification for High-Yield Production"
**作者**: Patel, R. et al.
**摘要**: 开发了一种新型亲和层析方法，显著提高ROBLD3重组蛋白的纯化效率，并验证其体外生物活性，适用于大规模药物筛选。
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**注意**：
- 上述文献为假设示例，实际研究中可能需替换“ROBLD3”为正确基因名(如ROBO1、ROBLD2等)。
- 建议通过PubMed、Google Scholar等平台，以关键词“ROBLD3 recombinant protein”或“ROBO重组蛋白”检索真实文献。
- 若研究领域涉及轴突导向或癌症，可扩展搜索Slit-Robo信号通路相关重组蛋白研究。
**Background of ROBLD3 Recombinant Protein**
ROBLD3 (Recombinant OB-fold Domain-containing Ligand 3) is a synthetic protein engineered to mimic the functional properties of the native ROBLD3 protein, which is characterized by its OB-fold (oligonucleotide/oligosaccharide-binding fold) structural motif. This conserved domain is commonly associated with nucleic acid binding, protein-protein interactions, and molecular recognition processes. The recombinant version is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling scalable and high-purity yields for research and therapeutic applications.
Native ROBLD3 is implicated in critical cellular pathways, including DNA repair, transcriptional regulation, and signal transduction. Its OB-fold domain facilitates interactions with DNA, RNA, or other proteins, suggesting roles in genome stability and cellular stress responses. Dysregulation of ROBLD3 has been linked to diseases such as cancer and neurodegenerative disorders, making it a target for mechanistic studies and drug development.
The recombinant form retains these biological activities while offering enhanced stability and reduced immunogenicity, achieved through advanced protein engineering techniques like codon optimization or site-directed mutagenesis. It is often tagged with affinity markers (e.g., His-tag, FLAG-tag) to simplify purification and detection in experimental workflows.
ROBLD3 recombinant protein is widely utilized in *in vitro* assays, structural studies (e.g., X-ray crystallography), and functional screens to elucidate its molecular interactions. Additionally, it serves as a potential therapeutic agent or diagnostic tool, particularly in oncology, where modulating its activity could influence tumor progression or treatment resistance. Ongoing research continues to explore its multifaceted roles in cellular homeostasis and disease pathology.
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