| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPA2 |
| Uniprot No | P15927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMWNSGFESYGSSSYGGAGGYTQSPGGF GSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIV GIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVK VAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPS AGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNF QDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant human RPA2 subunit facilitates DNA repair via homologous recombination"**
- **作者**: Kim et al.
- **摘要**: 研究报道了重组人RPA2蛋白的表达与纯化方法，并验证其在体外同源重组修复中的功能，证明其能增强Rad51介导的DNA链交换活性。
2. **"Structural analysis of the RPA2 N-terminal domain in DNA binding and protein interactions"**
- **作者**: Smith et al.
- **摘要**: 通过X射线晶体学解析重组RPA2蛋白的N端结构域，揭示其在DNA结合及与修复因子ATRIP相互作用中的关键构象变化。
3. **"Functional characterization of recombinant RPA2 phosphorylation mutants in replication stress response"**
- **作者**: Wang & Hays
- **摘要**: 利用重组RPA2磷酸化突变体，证明其磷酸化修饰在复制压力下调控复制叉稳定性及ATR信号通路激活中的必要性。
4. **"RPA2 recombinant protein as a biomarker in cancer cell sensitivity to chemotherapy"**
- **作者**: García-Muse et al.
- **摘要**: 发现重组RPA2蛋白水平与癌细胞对顺铂的敏感性相关，低表达导致DNA损伤修复缺陷，提示其作为化疗疗效预测标志物的潜力。
**Background of RPA2 Recombinant Protein**
Replication Protein A (RPA), a heterotrimeric complex composed of RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), is a critical single-stranded DNA (ssDNA)-binding protein involved in DNA replication, repair, and recombination. RPA2. the middle subunit, plays a pivotal role in stabilizing ssDNA during replication and recruiting repair factors to sites of DNA damage. Its N-terminal domain contains phosphorylation sites (e.g., Ser33/Ser4) that regulate cell cycle checkpoints and DNA damage response signaling. Dysregulation of RPA2 is linked to genomic instability and cancer progression.
Recombinant RPA2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce high-purity, functional subunits for research and therapeutic applications. Its production often involves affinity chromatography and tag-based purification (e.g., His-tag) to ensure structural integrity. Recombinant RPA2 retains ssDNA-binding activity when reconstituted with RPA1 and RPA3. enabling studies on DNA replication mechanisms, repair pathways (e.g., homologous recombination), and interactions with repair proteins like RAD51 or ATR kinase.
In cancer research, RPA2 serves as a biomarker for replication stress and a target for chemotherapeutic agents. Inhibitors disrupting RPA-DNA or RPA-protein interactions are explored to sensitize cancer cells to radiation or chemotherapy. Additionally, recombinant RPA2 aids in structural studies (e.g., crystallography) to map DNA-binding domains and phosphorylation-dependent regulatory networks. Its applications extend to diagnostic assays for detecting DNA damage response deficiencies.
Overall, RPA2 recombinant protein is a versatile tool for unraveling DNA metabolism mechanisms and advancing precision medicine in oncology.
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