| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL30 |
| Uniprot No | P62888 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK |
| 预测分子量 | 39.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL30重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: *"Expression and Purification of Recombinant Human RPL30 in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效表达人源RPL30重组蛋白的优化方法，利用His标签亲和层析纯化，并验证了重组蛋白的核糖体结合活性。
2. **标题**: *"Structural Analysis of RPL30 Reveals RNA-Binding Motifs Critical for Ribosome Assembly"*
**作者**: Lee J, et al.
**摘要**: 通过重组RPL30蛋白的晶体结构解析，鉴定了其与28S rRNA结合的特定结构域，揭示了RPL30在核糖体大亚基组装中的关键作用。
3. **标题**: *"Functional Characterization of Recombinant RPL30 in Zebrafish Development"*
**作者**: Chen H, et al.
**摘要**: 利用重组RPL30蛋白在斑马鱼模型中研究其功能，发现RPL30缺失导致胚胎发育异常，提示其在翻译调控和细胞增殖中的必要性。
4. **标题**: *"RPL30 Recombinant Protein as a Potential Biomarker in Breast Cancer"*
**作者**: Wang Y, et al.
**摘要**: 研究发现乳腺癌细胞中RPL30重组蛋白表达水平显著升高，通过体外实验证实其通过调控mTOR通路促进肿瘤细胞侵袭。
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**提示**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台以关键词“RPL30 recombinant protein”或“RPL30 expression”检索近年研究。
The RPL30 recombinant protein is derived from the ribosomal protein L30 (RPL30), a key component of the 60S subunit of eukaryotic ribosomes. As part of the ribosome, RPL30 plays a fundamental role in protein synthesis by facilitating mRNA decoding and peptide bond formation during translation. Beyond its canonical role, RPL30 has been implicated in extra-ribosomal functions, including interactions with RNA, regulation of cell cycle progression, and potential involvement in stress responses. Dysregulation of RPL30 expression has been associated with certain cancers and developmental disorders, highlighting its biological significance.
Recombinant RPL30 is typically produced using heterologous expression systems, such as E. coli or mammalian cell lines, to ensure high purity and functional activity. The protein is often engineered with affinity tags (e.g., His-tag) for simplified purification and detection. Structural studies reveal that RPL30 contains conserved domains critical for ribosome assembly and RNA binding, with a molecular weight of approximately 12-15 kDa depending on the species and tag configuration.
In research, recombinant RPL30 serves as a vital tool for investigating ribosome biogenesis, translational control mechanisms, and its non-ribosomal roles in cellular processes. It is also used to study interactions with other ribosomal proteins, RNA molecules, or potential inhibitors in drug discovery contexts. Quality control of the recombinant protein typically involves SDS-PAGE, western blotting, and functional assays to verify its binding or enzymatic activity. Its applications extend to disease models exploring ribosomopathies and cancer biology, where altered ribosome function contributes to pathogenesis.
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