| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL26 |
| Uniprot No | P61254 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKFNPFV TSDRSKNRKR HFNAPSHIRR KIMSSPLSKE LRQKYNVRSM PIRKDDEVQV VRGHYKGQQI GKVVQVYRKK YVIYIERVQR EKANGTTVHV GIHPSKVVIT RLKLDKDRKK ILERKAKSRQ VGKEKGKYKE ETIEKMQE |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL26重组蛋白的3篇虚构参考文献示例(仅供格式参考，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Expression and Purification of Recombinant Human RPL26 in Escherichia coli"
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达重组人源RPL26蛋白的优化方法，采用His标签亲和层析技术实现纯化。纯化的RPL26蛋白经Western blot和质谱验证，可用于后续结构及功能研究。
2. **文献名称**: "RPL26 Regulates mRNA Translation Fidelity by Binding to 28S rRNA"
**作者**: Lee S, Kim JH.
**摘要**: 通过体外重组RPL26蛋白与核糖体复合物的共结晶实验，发现RPL26通过结合28S rRNA的特定结构域调控翻译准确性，敲低RPL26导致哺乳动物细胞中错译率显著升高。
3. **文献名称**: "Role of Recombinant RPL26 in p53-Mediated Stress Response"
**作者**: Chen L, et al.
**摘要**: 利用重组RPL26蛋白的体外结合实验，证明其直接与p53 mRNA 5'UTR结合并增强翻译效率，在DNA损伤条件下促进肿瘤细胞凋亡，提示RPL26在应激反应中的调控作用。
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**注意**：以上内容为模拟示例，实际文献请通过PubMed、Web of Science等平台检索关键词"RPL26 recombinant protein"或"RPL26 AND (expression/purification/function)"获取。真实文献可能聚焦于RPL26在核糖体组装、翻译调控或疾病(如癌症)中的分子机制。
RPL26 (Ribosomal Protein L26) is a component of the 60S large ribosomal subunit, playing a critical role in protein synthesis by facilitating mRNA binding and translation fidelity. As part of the ribosome, it contributes to the structural stability of the complex and interacts with rRNA and other ribosomal proteins. Beyond its canonical role, RPL26 has been implicated in translational control under stress conditions, selectively regulating the expression of specific mRNAs, such as those encoding stress-response proteins or cell cycle regulators.
Recombinant RPL26 protein is engineered for experimental studies, typically produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modifications. Its recombinant form enables researchers to investigate ribosome biogenesis, protein-RNA interactions, and mechanisms of translational regulation. Notably, RPL26 has been linked to cancer biology, where its dysregulation affects tumor progression by modulating oncogene translation. Studies also highlight its interaction with the p53-MDM2 pathway, suggesting roles in stress-induced cell cycle arrest or apoptosis.
In drug discovery, recombinant RPL26 serves as a tool for screening compounds targeting ribosome function or stress-response pathways. Its applications extend to structural biology (cryo-EM studies) and diagnostics, as altered RPL26 expression correlates with certain cancers. Quality-controlled batches are validated via Western blot, mass spectrometry, and functional assays to ensure biological activity. Ongoing research continues to unravel its moonlighting functions beyond ribosome assembly, emphasizing its therapeutic and mechanistic relevance.
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