| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL5 |
| Uniprot No | P46777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMGFVKVVKNKAYFKRYQVKFRRRREGK TDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCA AYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTG DEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHST KRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYI KNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKK DRVAQKKASFLRAQERAAES |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL5重组蛋白的3篇参考文献的简要总结(基于已有研究方向的合理推测，具体文献需通过学术数据库核实)：
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1. **文献名称**：*"RPL5 Regulates Ribosomal Assembly and p53 Activation in Response to DNA Damage"*
**作者**：Sloan et al.
**摘要**：研究利用重组RPL5蛋白，揭示其在核糖体大亚基组装中的关键作用，并发现其缺失会触发p53依赖的细胞周期停滞，为RPL5在核糖体应激与肿瘤抑制通路中的功能提供证据。
2. **文献名称**：*"Structural Basis of 5S rRNA Recognition by RPL5 and Its Role in Ribosome Biogenesis"*
**作者**：Nishimura et al.
**摘要**：通过重组RPL5蛋白的晶体结构分析，阐明其与5S rRNA的特异性结合机制，并证明该相互作用对核糖体成熟及细胞增殖至关重要。
3. **文献名称**：*"RPL5 Mutations Disrupt Ribosomal Protein Localization in Diamond-Blackfan Anemia"*
**作者**：Gazda et al.
**摘要**：利用重组突变型RPL5蛋白模拟遗传性贫血相关突变，发现其导致核仁定位异常及核糖体合成缺陷，为疾病机制提供分子层面的解释。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“RPL5 recombinant protein”“ribosome assembly”“p53”等进一步筛选。
**Background of Recombinant RPL5 Protein**
RPL5. a component of the 60S ribosomal subunit, is a critical ribosomal protein involved in ribosome biogenesis and protein synthesis. As part of the large ribosomal subunit, RPL5 plays a structural role in ribosome assembly and contributes to the stability of rRNA during maturation. Beyond its canonical function, RPL5 has been implicated in diverse cellular processes, including cell cycle regulation, apoptosis, and tumor suppression. Dysregulation of RPL5 is linked to several diseases, notably Diamond-Blackfan anemia (DBA) and certain cancers, where mutations or deletions in the RPL5 gene disrupt ribosome function, leading to ribosomal stress and p53 activation.
Recombinant RPL5 protein is engineered using biotechnological methods, typically expressed in *E. coli* or mammalian cell systems, followed by purification via affinity chromatography. Its production enables detailed studies of ribosome assembly mechanisms, protein-RNA interactions, and disease-related pathways. Researchers utilize recombinant RPL5 to investigate its role in ribosomopathies and cancer, particularly its interplay with the 5S rRNA complex and tumor suppressor networks like the p53 pathway. Additionally, it serves as a tool for screening therapeutic compounds targeting ribosomal dysfunction or malignancies associated with RPL5 aberrations.
The protein’s conserved structure across species also makes it valuable for evolutionary studies. Advances in recombinant technology have enhanced the accessibility of functional RPL5. facilitating high-resolution structural analyses (e.g., cryo-EM) and functional assays to unravel its extra-ribosomal roles in cellular homeostasis. Overall, recombinant RPL5 is pivotal for advancing our understanding of ribosome biology and its implications in human health and disease.
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