| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS12 |
| Uniprot No | P25398 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAEEGIA AGGVMDVNTA LQEVLKTALI HDGLARGIRE AAKALDKRQA HLCVLASNCD EPMYVKLVEA LCAEHQINLI KVDDNKKLGE WVGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS12重组蛋白的3篇模拟参考文献示例，供参考：
---
1. **文献名称**：*Structural and functional characterization of recombinant human RPS12 in ribosome assembly*
**作者**：Li, X., Chen, J., & Wang, Y.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组人源RPS12蛋白，并利用冷冻电镜技术分析其在小亚基核糖体组装中的构象变化。实验表明，RPS12通过与18S rRNA结合调控核糖体成熟，缺失该蛋白会导致翻译效率显著下降。
2. **文献名称**：*RPS12 mutations alter antibiotic susceptibility via recombinant protein-mediated ribosomal stalling*
**作者**：Smith, T.K., et al.
**摘要**：通过构建携带RPS12突变的原核重组蛋白，揭示了其与氨基糖苷类抗生素耐药性的关联。突变体RPS12改变了核糖体解码中心的构象，导致错误翻译率增加，进而影响细菌对抗生素的敏感性。
3. **文献名称**：*Recombinant RPS12 as a potential biomarker in hepatocellular carcinoma progression*
**作者**：Guo, R., et al.
**摘要**：在肝癌细胞系中过表达重组RPS12蛋白，发现其通过激活mTOR通路促进肿瘤细胞增殖和迁移。临床样本分析显示，RPS12的高表达与患者预后不良显著相关。
---
**注**：以上文献为模拟示例，实际研究中建议通过PubMed、Web of Science等平台检索真实发表的论文。
**Background of RPS12 Recombinant Protein**
RPS12 (Ribosomal Protein S12) is a component of the 40S ribosomal subunit, playing a critical role in mRNA decoding and translational fidelity during protein synthesis. As part of the conserved ribosomal machinery, RPS12 contributes to ribosome assembly, stability, and interaction with elongation factors. Mutations in RPS12 have been linked to human diseases, including Diamond-Blackfan anemia (DBA), a rare bone marrow failure disorder, underscoring its importance in hematopoiesis and cellular homeostasis.
Recombinant RPS12 protein is engineered for in vitro studies to dissect its structural and functional roles. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), the protein is purified using affinity tags (e.g., His-tag) for high yield and purity. Its recombinant form enables research on ribosome biogenesis, translational regulation, and disease mechanisms. For example, studies using recombinant RPS12 have explored how specific mutations impair ribosome assembly or alter interactions with rRNA and other ribosomal proteins.
Additionally, RPS12 has been implicated in bacterial antibiotic resistance; in *Mycobacterium tuberculosis*, mutations in RPS12 homologs correlate with resistance to streptomycin. This highlights its potential as a target for antimicrobial drug development. Structural analyses (e.g., cryo-EM) leveraging recombinant RPS12 have advanced understanding of ribosome architecture and dynamics.
Overall, recombinant RPS12 serves as a vital tool for elucidating ribosome-related pathologies, antibiotic resistance mechanisms, and fundamental processes in gene expression.
×
