| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS16 |
| Uniprot No | P62249 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPSKGPL QSVQVFGRKK TATAVAHCKR GNGLIKVNGR PLEMIEPRTL QYKLLEPVLL LGKERFAGVD IRVRVKGGGH VAQIYAIRQS ISKALVAYYQ KYVDEASKKE IKDILIQYDR TLLVADPRRC ESKKFGGPGA RARYQKSYR |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS16重组蛋白的3篇参考文献，包含文献名称、作者及简要摘要内容概括：
1. **文献名称**：*"Cloning, expression, and functional analysis of recombinant human RPS16 in ribosome biogenesis"*
**作者**：Zhang Y, et al.
**摘要**：研究通过克隆人源RPS16基因并在大肠杆菌中表达重组蛋白，验证其在核糖体组装中的关键作用，发现RPS16缺陷导致核糖体亚基异常聚集。
2. **文献名称**：*"Structural characterization of recombinant RPS16 from Arabidopsis thaliana and its role in stress response"*
**作者**：Lee S, Kim J.
**摘要**：利用X射线晶体学解析拟南芥RPS16重组蛋白结构，揭示其与mRNA结合的活性位点，并证明其在植物逆境响应中调控翻译效率。
3. **文献名称**：*"Functional interaction between RPS16 and p53: Implications for cancer progression"*
**作者**：Chen L, et al.
**摘要**：通过重组人RPS16蛋白与p53的体外结合实验，发现RPS16通过抑制p53泛素化稳定其表达，提示其在肿瘤发生中的潜在调控机制。
(注：以上为模拟文献，实际文献需通过PubMed或Google Scholar检索具体标题及作者。)
**Background of RPS16 Recombinant Protein**
Ribosomal protein S16 (RPS16) is a critical component of the 40S ribosomal subunit, playing a fundamental role in mRNA translation and ribosome biogenesis. As part of the small ribosomal subunit, RPS16 contributes to the structural integrity of the ribosome and facilitates the accurate decoding of genetic information during protein synthesis. It is highly conserved across eukaryotes, underscoring its essential role in cellular function.
The gene encoding RPS16 is located on human chromosome 19 and is ubiquitously expressed, reflecting its necessity in all cell types. Beyond its canonical role in translation, RPS16 has been implicated in extra-ribosomal functions, including cell cycle regulation, apoptosis, and interactions with viral RNAs, suggesting broader regulatory roles in cellular homeostasis. Dysregulation of RPS16 expression or mutations in its coding sequence have been linked to developmental disorders, such as Diamond-Blackfan anemia (DBA), a rare bone marrow failure syndrome characterized by defective ribosome assembly (ribosomopathy).
Recombinant RPS16 protein is engineered using heterologous expression systems (e.g., *E. coli* or yeast) to produce purified, functional protein for research applications. This recombinant form enables detailed studies of RPS16's molecular interactions, structural dynamics, and mechanisms underlying ribosome assembly and disease pathology. It is widely utilized in *in vitro* assays, structural biology (e.g., X-ray crystallography), and therapeutic target validation. Additionally, recombinant RPS16 serves as a tool to investigate its potential role in cancer, viral infections, and genetic disorders, offering insights into novel therapeutic strategies targeting ribosomal dysfunction.
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