| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS20 |
| Uniprot No | P60866 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAFKDTG KTPVEPEVAI HRIRITLTSR NVKSLEKVCA DLIRGAKEKN LKVKGPVRMP TKTLRITTRK TPCGEGSKTW DRFQMRIHKR LIDLHSPSEI VKQITSISIE PGVELIESTD AEPMDTEGQQ YTLRSVFESP GTCPF |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS20重组蛋白的模拟参考文献示例(注：文献信息为虚构，仅供格式参考)：
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1. **标题**: *Structural insights into human RPS20 in ribosome assembly*
**作者**: Smith A, et al.
**摘要**: 通过X射线晶体学解析了重组人源RPS20蛋白的三维结构，揭示了其与18S rRNA的结合位点，为核糖体小亚基组装机制提供了新见解。
2. **标题**: *High-yield expression and purification of recombinant RPS20 in E. coli*
**作者**: Zhang L, et al.
**摘要**: 开发了一种基于大肠杆菌的高效重组RPS20表达系统，优化纯化步骤获得高纯度蛋白，适用于体外功能研究与抗体开发。
3. **标题**: *RPS20 mutations drive hereditary colorectal cancer via impaired ribosome biogenesis*
**作者**: Johnson R, et al.
**摘要**: 发现RPS20基因突变通过破坏核糖体生物合成导致DNA修复缺陷，首次将RPS20异常与林奇综合征相关结直肠癌联系起来。
4. **标题**: *RPS20 interacts with viral RNA to promote enterovirus replication*
**作者**: Lee H, et al.
**摘要**: 证实重组RPS20蛋白与肠道病毒RNA直接结合，通过调控病毒翻译效率影响复制周期，为抗病毒靶点开发提供依据。
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*注：以上文献为示例性内容，实际研究中请通过PubMed或Google Scholar检索真实发表论文。*
RPS20 (ribosomal protein S20) is a component of the 40S ribosomal subunit, playing a critical role in mRNA translation and ribosome biogenesis. As part of the conserved ribosomal protein family, it facilitates the assembly and stability of ribosomal subunits, ensuring accurate protein synthesis. Beyond its canonical role, RPS20 has been implicated in extra-ribosomal functions, including DNA repair and apoptosis regulation, highlighting its multifaceted cellular importance.
Recombinant RPS20 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active forms for functional studies. Its recombinant version often includes affinity tags (e.g., His-tag) for simplified purification via chromatography. Researchers utilize this tool to investigate RPS20’s structural interactions within ribosomes, its binding partners, and its role in translational fidelity. Dysregulation of RPS20 has been linked to human diseases; for example, germline mutations are associated with hereditary colorectal cancer (Lynch-like syndrome) and Diamond-Blackfan anemia (DBA), a ribosomopathy. Recombinant RPS20 enables mechanistic studies of these pathologies, including how specific mutations impair ribosome assembly or promote oncogenesis.
Additionally, RPS20’s involvement in p53-dependent stress responses and its potential as a biomarker in cancers (e.g., colorectal, gastric) have spurred interest in therapeutic targeting. Studies using recombinant protein help elucidate post-translational modifications, subcellular localization, and signaling pathways influenced by RPS20. Its conserved sequence across eukaryotes also makes it a model for exploring ribosome evolution. Overall, recombinant RPS20 serves as a vital resource for dissecting ribosome biology, disease mechanisms, and translational control networks.
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