| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS3A |
| Uniprot No | P61247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1- 264aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMAVGKN KRLTKGGKKG AKKKVVDPFS KKDWYDVKAP AMFNIRNIGK TLVTRTQGTK IASDGLKGRV FEVSLADLQN DEVAFRKFKL ITEDVQGKNC LTNFHGMDLT RDKMCSMVKK WQTMIEAHVD VKTTDGYLLR LFCVGFTKKR NNQIRKTSYA QHQQVRQIRK KMMEIMTREV QTNDLKEVVN KLIPDSIGKD IEKACQSIYP LHDVFVRKVK MLKKPKFELG KLMELHGEGS SSGKATGDET GAKVERADGY EPPVQESV |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS3A重组蛋白的3篇参考文献的简要概括(注：以下为模拟示例，具体文献需根据实际数据库检索确认)：
---
1. **文献名称**: "Expression and Purification of Recombinant Ribosomal Protein S3A (RPS3A) in a Prokaryotic System"
**作者**: Zhang Y, et al.
**摘要**: 研究报道了通过原核表达系统(大肠杆菌)成功克隆并高效表达人源RPS3A蛋白，优化了纯化条件，并验证了其与核糖体RNA结合的活性。
---
2. **文献名称**: "Functional Analysis of Recombinant RPS3A in DNA Damage Repair"
**作者**: Lee S, Kim J.
**摘要**: 通过重组RPS3A蛋白的体外实验，揭示了其在DNA损伤修复中的双重功能，证明RPS3A不仅参与核糖体组装，还可能通过调控特定信号通路影响细胞应激反应。
---
3. **文献名称**: "RPS3A Recombinant Protein as a Potential Biomarker in Hepatocellular Carcinoma"
**作者**: Wang X, et al.
**摘要**: 研究发现肝癌细胞中RPS3A表达异常升高，通过重组蛋白制备及功能实验，证实其通过调控AKT/mTOR通路促进肿瘤增殖，提示其作为治疗靶点的潜力。
---
如需具体文献，建议在PubMed或Web of Science中检索关键词“Recombinant RPS3A protein”或“RPS3A expression”获取最新研究。
**Background of RPS3A Recombinant Protein**
RPS3A (ribosomal protein S3A) is a component of the 40S ribosomal subunit, playing a fundamental role in mRNA translation and ribosome biogenesis. As part of the ribosome, it contributes to the decoding of genetic information by stabilizing rRNA structure and facilitating interactions between ribosomal subunits. Beyond its canonical role in protein synthesis, RPS3A has been implicated in diverse cellular processes, including apoptosis regulation, DNA repair, and viral infection responses. Studies suggest it may act as a multifunctional protein, with roles in modulating stress signaling pathways and interacting with non-ribosomal proteins to influence cell survival.
The recombinant RPS3A protein is engineered using expression systems like *E. coli* or mammalian cells, enabling high-purity production for functional studies. Its recombinant form retains critical structural features, such as conserved domains for ribosome binding and nuclear localization signals, allowing researchers to explore its extra-ribosomal functions. For instance, RPS3A has been linked to cancer progression due to its overexpression in certain tumors, where it may enhance cell proliferation or suppress apoptosis. Additionally, it interacts with viral proteins, suggesting a role in host-pathogen interactions.
Research on RPS3A recombinant protein aids in elucidating its molecular mechanisms, including post-translational modifications (e.g., phosphorylation) that regulate its activity. It is also utilized in structural biology (e.g., X-ray crystallography) to resolve ribosome architecture and in drug discovery platforms targeting ribosome-related diseases. Understanding RPS3A's dual roles in translation and cellular stress responses could provide insights into therapeutic strategies for cancers, neurodegenerative disorders, or viral infections. Overall, RPS3A exemplifies the expanding recognition of ribosomal proteins as dynamic players in both homeostasis and disease.
×
