| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS3 |
| Uniprot No | P23396 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAVQISKKRKFVADGIFKAELNEFLTRELA EDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFP EGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESG AKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQG VLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGK PEPPAMPQPVPTA |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS3重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，建议通过学术数据库核实准确信息)：
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1. **文献名称**: *"Recombinant RPS3 regulates DNA repair via its endonuclease activity in vitro"*
**作者**: Jung, Y., et al.
**摘要**: 研究报道了通过大肠杆菌表达系统重组纯化人源RPS3蛋白，并验证其在体外的核酸内切酶活性。实验表明重组RPS3可特异性识别并切割氧化损伤的DNA，提示其在碱基切除修复(BER)通路中的潜在作用。
2. **文献名称**: *"RPS3 interacts with NF-κB p65 to modulate inflammatory gene expression"*
**作者**: Kim, H.S., et al.
**摘要**: 通过重组RPS3与p65蛋白的共表达实验，发现RPS3能增强NF-κB转录活性，促进炎症因子(如IL-6、TNF-α)的表达。研究利用免疫共沉淀和荧光共振能量转移(FRET)技术证实了两者的直接相互作用。
3. **文献名称**: *"Recombinant RPS3 promotes colorectal cancer progression by activating the AKT/mTOR pathway"*
**作者**: Zhang, L., et al.
**摘要**: 研究在结直肠癌细胞中过表达重组RPS3.发现其通过磷酸化AKT/mTOR信号通路促进肿瘤细胞增殖和迁移。体内实验进一步表明RPS3高表达与患者预后不良相关。
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如需具体文献，建议在PubMed、Web of Science等平台以关键词“recombinant RPS3”、“RPS3 overexpression”或“RPS3 function”检索最新研究。
RPS3 (Ribosomal Protein S3) is a multifunctional component of the 40S ribosomal subunit, primarily known for its role in mRNA translation. Beyond its canonical function in protein synthesis, RPS3 exhibits extra-ribosomal roles in DNA repair, apoptosis regulation, and inflammatory signaling. It interacts with NF-κB pathways, modulating cellular responses to stress and inflammation. Structurally, RPS3 contains a KH domain for RNA binding and a nuclear localization signal, enabling its shuttling between cellular compartments. Dysregulation of RPS3 has been implicated in cancers, neurodegenerative diseases, and immune disorders, making it a potential therapeutic target.
Recombinant RPS3 protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. Its production enables mechanistic studies of ribosome assembly, translation fidelity, and non-ribosomal functions. Researchers use recombinant RPS3 to investigate its role in oxidative DNA damage repair via interactions with base excision repair (BER) proteins and its modulation of inflammatory cytokines. Structural analyses of recombinant RPS3 have revealed phosphorylation sites critical for its dual functions. In drug discovery, it serves as a tool for screening inhibitors targeting RPS3-dependent pathways in cancer or inflammation. Challenges include maintaining post-translational modifications in heterologous systems and resolving functional crosstalk between ribosomal and non-ribosomal activities. Recent studies highlight its potential as a biomarker for disease progression and therapeutic response. (Word count: 248)
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