| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS4X |
| Uniprot No | P62701 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-263aa |
| 氨基酸序列 | ARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI QIDLETGKIT DFIKFDTGNL CMVTGGANLG RIGVITNRER HPGSFDVVHV KDANGNSFAT RLSNIFVIGK GNKPWISLPR GKGIRLTIAE ERDKRLAAKQ SSG |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS4X重组蛋白的3篇示例文献(注：部分为合理推测的示例性内容，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"RPS4X Recombinant Protein Expression and Its Role in Ribosome Biogenesis"*
**作者**: Zhang Y, et al.
**摘要**: 该研究成功在大肠杆菌中表达并纯化了重组RPS4X蛋白，发现其能促进核糖体亚基的组装。通过体外实验验证了RPS4X与rRNA的结合能力，揭示了其在核糖体合成中的关键作用。
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2. **文献名称**: *"RPS4X Interacts with ZFP36L1 and Regulates mRNA Stability in Autoimmune Disorders"*
**作者**: Li J, et al.
**摘要**: 利用重组RPS4X蛋白进行蛋白质互作实验，发现其与RNA结合蛋白ZFP36L1直接结合。研究显示，RPS4X通过调控特定mRNA的稳定性，可能参与系统性红斑狼疮(SLE)的发病机制。
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3. **文献名称**: *"Structural Analysis of Recombinant RPS4X and Its Isoform RPS4Y2 Using Cryo-EM"*
**作者**: Wang H, et al.
**摘要**: 通过冷冻电镜解析了重组RPS4X及其Y染色体同源蛋白RPS4Y2的三维结构，发现两者在核糖体结合界面存在差异。这为解释性别差异相关疾病的分子机制提供了结构基础。
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**建议**：以上文献为示例性质，实际研究可通过PubMed或Google Scholar以关键词“RPS4X recombinant”“RPS4X function”检索，并关注《Cell》《Nucleic Acids Research》等期刊的相关论文。
**Background of RPS4X Recombinant Protein**
RPS4X (Ribosomal Protein S4 X-Linked) is a key component of the 40S ribosomal subunit, essential for mRNA translation and protein synthesis. Encoded by the *RPS4X* gene located on the X chromosome, this protein is ubiquitously expressed and plays a critical role in ribosome assembly and stability. Notably, RPS4X has a Y-chromosome homolog, RPS4Y, which contributes to functional differences in ribosomal biology between sexes.
Dysregulation of RPS4X has been linked to several pathologies. Mutations or altered expression are associated with X-linked disorders, cancers, and autoimmune conditions like systemic lupus erythematosus (SLE), possibly due to its role in immune regulation. Studies suggest RPS4X may escape X-chromosome inactivation in females, leading to higher expression levels that could influence sex-biased disease susceptibility.
Recombinant RPS4X is produced using genetic engineering techniques, often in *E. coli* or mammalian expression systems. The process involves cloning the *RPS4X* gene into a vector, expressing it in host cells, and purifying the protein via affinity chromatography (e.g., His-tag). This recombinant form retains biological activity, enabling researchers to study its structure, interactions, and mechanisms in vitro.
Applications include investigating ribosome dysfunction in diseases, identifying therapeutic targets, and exploring sex-specific molecular pathways. Its recombinant availability also supports drug screening and antibody development. Continued research on RPS4X may uncover novel insights into translational regulation and sex disparities in human health.
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