| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RQCD1 |
| Uniprot No | Q92600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMHSLATAAPVPTTLAQVDREKIYQWINELS SPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEI VNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLY PFLHTVSKTRPFEYLRLTSLGVIGALVKTDEQEVINFLLTTEIIPLCLRI MESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQL SKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDT TTKRWLAQLVKNLQEGQVTDPRGIPLPPQ |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RQCD1重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际研究需查询数据库)：
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1. **文献名称**: "RQCD1 modulates DNA damage response via interacting with ATM kinase"
**作者**: Tanaka, K., et al.
**摘要**: 本研究阐明了RQCD1重组蛋白通过与ATM激酶相互作用，在DNA损伤修复中的调控机制。实验表明，RQCD1缺失导致细胞对辐射敏感性增加，提示其在基因组稳定性中的关键作用。
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2. **文献名称**: "Structural characterization of recombinant RQCD1 and its role in apoptosis"
**作者**: Chen, L., et al.
**摘要**: 通过X射线晶体学解析了RQCD1重组蛋白的三维结构，发现其C端结构域参与结合促凋亡蛋白Bax。功能实验证实RQCD1在细胞凋亡通路中起正向调控作用。
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3. **文献名称**: "RQCD1 as a potential biomarker in breast cancer: Recombinant protein-based analysis"
**作者**: Gupta, R., & Patel, S.
**摘要**: 利用重组RQCD1蛋白开发了新型ELISA检测方法，发现其在乳腺癌患者血清中表达显著升高。研究提示RQCD1可能作为癌症诊断或预后的生物标志物。
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**备注**：实际研究中RQCD1相关文献较少，建议通过PubMed或Web of Science以“RQCD1 recombinant protein”为关键词检索最新成果，并注意名称拼写准确性(如是否为RCD1或类似缩写)。
**Background of RQCD1 Recombinant Protein**
RQCD1 (Required for Cell Differentiation 1), also known as CRLD2 or CCDC47. is a conserved eukaryotic protein implicated in diverse cellular processes, including transcriptional regulation, RNA metabolism, and cell cycle control. It was initially identified for its role in promoting cell differentiation and maintaining cellular homeostasis. Structurally, RQCD1 contains coiled-coil domains, which mediate protein-protein interactions, and a nuclear localization signal, suggesting its involvement in nuclear functions. Studies highlight its interaction with key signaling pathways, such as the Wnt/β-catenin and TGF-β pathways, where it acts as a modulator of gene expression and cellular responses.
The recombinant RQCD1 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This engineered protein retains functional domains critical for binding partners like Dishevelled (Dvl) in Wnt signaling or Smad proteins in TGF-β signaling, enabling its use in mechanistic studies. Researchers employ recombinant RQCD1 to investigate its role in diseases such as cancer, where dysregulation of differentiation and proliferation pathways is common. For instance, RQCD1 overexpression or knockdown models reveal its influence on tumor progression, metastasis, and chemoresistance.
Additionally, RQCD1’s involvement in RNA splicing and stability links it to neurological disorders and developmental anomalies. Its recombinant form facilitates *in vitro* assays, including pull-down experiments, enzymatic activity studies, and structural analyses. Quality-controlled batches ensure consistency in research applications, supporting advancements in understanding RQCD1’s molecular interactions and therapeutic potential. Overall, recombinant RQCD1 serves as a vital tool for dissecting its multifaceted roles in health and disease.
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