| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRAGC |
| Uniprot No | Q9HB90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSLQYG AEETPLAGSY GAADSFPKDF GYGVEEEEEE AAAAGGGVGA GAGGGCGPGG ADSSKPRILL MGLRRSGKSS IQKVVFHKMS PNETLFLEST NKIYKDDISN SSFVNFQIWD FPGQMDFFDP TFDYEMIFRG TGALIYVIDA QDDYMEALTR LHITVSKAYK VNPDMNFEVF IHKVDGLSDD HKIETQRDIH QRANDDLADA GLEKLHLSFY LTSIYDHSIF EAFSKVVQKL IPQLPTLENL LNIFISNSGI EKAFLFDVVS KIYIATDSSP VDMQSYELCC DMIDVVIDVS CIYGLKEDGS GSAYDKESMA IIKLNNTTVL YLKEVTKFLA LVCILREESF ERKGLIDYNF HCFRKAIHEV FEVGVTSHRS CGHQTSASSL KALTHNGTPR NAI |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RRAGC重组蛋白的3篇示例参考文献(注：以下文献信息为示例性质，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Recombinant expression and functional characterization of human RRAGC in mTORC1 signaling"
**作者**: Li, X., et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组人源RRAGC蛋白，证实其GTPase活性对mTORC1通路的调控作用。通过体外结合实验，发现RRAGC与Raptor蛋白的相互作用依赖于其GTP结合状态。
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2. **文献名称**: "Structural insights into RRAGC dimerization and lysosomal recruitment by the Ragulator complex"
**作者**: Sancak, Y., et al.
**摘要**: 通过冷冻电镜技术解析了重组RRAGC与Ragulator复合体的三维结构，揭示了RRAGC二聚化及其在溶酶体膜定位中的关键构象变化，为mTORC1营养感应机制提供结构基础。
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3. **文献名称**: "Disease-associated mutations in RRAGC disrupt GTP-binding and impair cellular metabolic homeostasis"
**作者**: Kim, J., & Lee, M.
**摘要**: 研究利用重组突变型RRAGC蛋白，证明某些癌症相关突变(如S75L)导致GTP结合能力丧失，进而抑制mTORC1信号传导，影响细胞生长和自噬过程。
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**建议**：可通过PubMed或Google Scholar搜索关键词“RRAGC recombinant”、“RRAGC mTOR”或“RRAGC structure”获取最新研究。实际文献需根据具体研究方向筛选。
RRAGC (Ras-related GTP-binding protein C) is a member of the Rag GTPase family, which plays a critical role in nutrient-sensing signaling pathways, particularly the mechanistic target of rapamycin complex 1 (mTORC1) pathway. As a small GTPase, RRAGC cycles between GTP-bound (active) and GDP-bound (inactive) states, forming heterodimers with RagA/B or RagC/D to regulate mTORC1 localization and activity. This process is essential for cellular responses to amino acid availability, energy status, and growth signals, influencing processes like protein synthesis, autophagy, and metabolic homeostasis. Dysregulation of RRAGC-associated pathways is linked to diseases such as cancer, metabolic disorders, and neurodegenerative conditions.
Recombinant RRAGC proteins are engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional forms of the protein for research. These proteins retain GTP/GDP-binding capacity and interaction properties with binding partners like the Ragulator complex or GATOR1/GATOR2 regulators. Their production enables mechanistic studies on mTORC1 activation, nutrient signaling crosstalk, and mutations found in diseases (e.g., cancer-associated RRAGC variants). Recombinant RRAGC is also used in drug discovery to screen inhibitors targeting mTOR pathway components, with potential therapeutic applications. Structural studies using recombinant proteins have advanced understanding of Rag GTPase conformational changes during nutrient sensing. As a key node in cellular metabolism, RRAGC remains a focus for elucidating disease mechanisms and developing targeted therapies.
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