| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD13 |
| Uniprot No | Q5VXD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-122aa |
| 氨基酸序列 | MANSLLEGVF AEVKEPCSLP MLSVDMENKE NGSVGVKNSM ENGRPPDPAD WAVMDVVNYF RTVGFEEQAS AFQEQEIDGK SLLLMTRNDV LTGLQLKLGP ALKIYEYHVK PLQTKHLKNN SS |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAMD13重组蛋白的示例性参考文献(注：SAMD13相关研究较为有限，以下内容为模拟概括，建议通过学术数据库核实最新文献)：
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1. **文献名称**: *"Cloning and Expression of Recombinant SAMD13 Protein in E. coli for Functional Studies"*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了SAMD13基因的克隆及在大肠杆菌中的重组表达，优化了表达条件并通过亲和层析纯化获得高纯度蛋白。初步功能分析表明SAMD13可能通过其SAM结构域参与细胞信号转导。
2. **文献名称**: *"Structural Characterization of SAMD13 and Its Interaction with Lipid Membranes"*
**作者**: Patel R, et al.
**摘要**: 利用X射线晶体学解析了重组SAMD13蛋白的SAM结构域三维结构，并发现其与细胞膜脂质成分存在特异性结合，提示其在膜相关生物学过程中的潜在作用。
3. **文献名称**: *"SAMD13 Regulates Autophagy via mTOR Signaling Pathway: Insights from Recombinant Protein Overexpression Models"*
**作者**: Kim S, et al.
**摘要**: 通过在HEK293细胞中过表达重组SAMD13蛋白，发现其可通过抑制mTOR通路增强自噬活性，为研究SAMD13在代谢疾病中的功能提供了实验依据。
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**建议**：由于SAMD13的研究尚处于早期阶段，上述内容为模拟示例。建议通过PubMed或Google Scholar以“SAMD13 recombinant protein”“SAMD13 expression”等关键词检索近期文献，或关注相关基因家族(如SAM结构域蛋白)的功能研究扩展信息。
**Background of SAMD13 Recombinant Protein**
SAMD13 (Sterile Alpha Motif Domain-containing protein 13) is a member of the SAM domain-containing protein family, characterized by the presence of the evolutionarily conserved SAM domain. This ~70-residue motif facilitates protein-protein and protein-lipid interactions, playing roles in diverse cellular processes, including signal transduction, transcriptional regulation, and membrane remodeling. SAMD13. though less studied compared to other SAM domain proteins, is hypothesized to participate in intracellular signaling pathways, potentially influencing cell proliferation, apoptosis, or immune responses. Its exact physiological role remains under investigation, but structural homology suggests involvement in organizing molecular complexes or modulating enzymatic activity.
Recombinant SAMD13 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection. Studies leveraging SAMD13 recombinant protein aim to elucidate its interactome, structural features, and mechanistic contributions to cellular pathways. For instance, it may serve as a tool to identify binding partners via pull-down assays or to probe its role in diseases linked to SAM domain dysregulation, such as cancer or autoimmune disorders.
Emerging interest in SAMD13 also stems from its potential as a therapeutic target or diagnostic biomarker. However, further functional and structural analyses are required to define its precise biological significance and translational relevance. Current research focuses on characterizing its post-translational modifications, subcellular localization, and tissue-specific expression patterns to unravel its context-dependent functions.
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